CPT® Code 0564T

Deleted

Oncology, chemotherapeutic drug cytotoxicity assay of cancer stem cells (CSCs), from cultured CSCs and primary tumor cells, categorical drug response reported based on percent of cytotoxicity observed, a minimum of 14 drugs or drug combinations

The CPT® Code 0564T refers to an oncology procedure known as a chemotherapeutic drug cytotoxicity assay specifically designed to evaluate the response of cancer stem cells (CSCs) and primary tumor cells to various chemotherapy agents. This assay is crucial in determining how effective specific chemotherapeutic drugs or combinations thereof are in inducing cell death, which is quantified as cytotoxicity. Cytotoxicity, in this context, is defined as the degree of cell death that occurs when tumor cells and CSCs are exposed to chemotherapy treatments. The assay utilizes unfixed tumor tissue, which is cultured to isolate and enrich the cancer stem cells. The cultured tumor cells and CSCs are then placed into separate 96-well plates, where they are treated with a minimum of 14 different chemotherapy drugs or combinations of these drugs. Over a period of 48 hours, the metabolic activity of the treated cells is monitored using a colorimetric assay, which compares the treated cells to a control group of untreated cells. The results of the cytotoxicity assay are expressed as a percentage, indicating the proportion of cells that have died as a result of the drug treatment. A response to treatment is characterized by a cytotoxicity level ranging from 60% to 100%, while a lack of response is indicated by cytotoxicity levels below 10% to 30%.