© Copyright 2026 American Medical Association. All rights reserved.
A biopsy of the testis is a medical procedure that involves the removal of a small sample of testicular tissue for diagnostic purposes. This procedure is categorized as an incisional biopsy, which means that a surgical incision is made to access the testis directly, allowing for the collection of a tissue sample. The primary goal of this biopsy is to evaluate abnormalities within the testis, such as masses or the absence of living sperm, which may be indicative of underlying conditions affecting male fertility or testicular health. Prior to the procedure, the skin over the intended incision site is thoroughly cleansed to minimize the risk of infection, and a local anesthetic is administered to ensure patient comfort during the operation. Depending on the clinical indication, the surgeon may expose a mass within the testis to obtain a sample or may collect tissue to investigate cases of azoospermia, where no sperm is detected in the semen. The collected tissue is then preserved in Bouin's fluid and sent to a laboratory for histological evaluation, which is essential for diagnosing potential testicular disorders or confirming the presence of sperm in cases of infertility.
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