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Official Description

Molecular pathology procedure, Level 6 (eg, analysis of 6-10 exons by DNA sequence analysis, mutation scanning or duplication/deletion variants of 11-25 exons, regionally targeted cytogenomic array analysis)

ABCD1 (ATP-binding cassette, sub-family D [ALD], member 1) (eg, adrenoleukodystrophy), full gene sequence

ACADS (acyl-CoA dehydrogenase, C-2 to C-3 short chain) (eg, short chain acyl-CoA dehydrogenase deficiency), full gene sequence

ACTA2 (actin, alpha 2, smooth muscle, aorta) (eg, thoracic aortic aneurysms and aortic dissections), full gene sequence

ACTC1 (actin, alpha, cardiac muscle 1) (eg, familial hypertrophic cardiomyopathy), full gene sequence

ANKRD1 (ankyrin repeat domain 1) (eg, dilated cardiomyopathy), full gene sequence

APTX (aprataxin) (eg, ataxia with oculomotor apraxia 1), full gene sequence

ARSA (arylsulfatase A) (eg, arylsulfatase A deficiency), full gene sequence

BCKDHA (branched chain keto acid dehydrogenase E1, alpha polypeptide) (eg, maple syrup urine disease, type 1A), full gene sequence

BCS1L (BCS1-like [S. cerevisiae]) (eg, Leigh syndrome, mitochondrial complex III deficiency, GRACILE syndrome), full gene sequence

BMPR2 (bone morphogenetic protein receptor, type II [serine/threonine kinase]) (eg, heritable pulmonary arterial hypertension), duplication/deletion analysis

CASQ2 (calsequestrin 2 [cardiac muscle]) (eg, catecholaminergic polymorphic ventricular tachycardia), full gene sequence

CASR (calcium-sensing receptor) (eg, hypocalcemia), full gene sequence

CDKL5 (cyclin-dependent kinase-like 5) (eg, early infantile epileptic encephalopathy), duplication/deletion analysis

CHRNA4 (cholinergic receptor, nicotinic, alpha 4) (eg, nocturnal frontal lobe epilepsy), full gene sequence

CHRNB2 (cholinergic receptor, nicotinic, beta 2 [neuronal]) (eg, nocturnal frontal lobe epilepsy), full gene sequence

COX10 (COX10 homolog, cytochrome c oxidase assembly protein) (eg, mitochondrial respiratory chain complex IV deficiency), full gene sequence

COX15 (COX15 homolog, cytochrome c oxidase assembly protein) (eg, mitochondrial respiratory chain complex IV deficiency), full gene sequence

CPOX (coproporphyrinogen oxidase) (eg, hereditary coproporphyria), full gene sequence

CTRC (chymotrypsin C) (eg, hereditary pancreatitis), full gene sequence

CYP11B1 (cytochrome P450, family 11, subfamily B, polypeptide 1) (eg, congenital adrenal hyperplasia), full gene sequence

CYP17A1 (cytochrome P450, family 17, subfamily A, polypeptide 1) (eg, congenital adrenal hyperplasia), full gene sequence

CYP21A2 (cytochrome P450, family 21, subfamily A, polypeptide2) (eg, steroid 21-hydroxylase isoform, congenital adrenal hyperplasia), full gene sequence

Cytogenomic constitutional targeted microarray analysis of chromosome 22q13 by interrogation of genomic regions for copy number and single nucleotide polymorphism (SNP) variants for chromosomal abnormalities

(When performing cytogenomic [genome-wide] analysis for constitutional chromosomal abnormalities, see 81228, 81229, 81349)

(Do not report analyte-specific molecular pathology procedures separately when the specific analytes are included as part of the microarray analysis of chromosome 22q13)

(Do not report 88271 when performing cytogenomic microarray analysis)

DBT (dihydrolipoamide branched chain transacylase E2) (eg, maple syrup urine disease, type 2), duplication/deletion analysis

DCX (doublecortin) (eg, X-linked lissencephaly), full gene sequence

DES (desmin) (eg, myofibrillar myopathy), full gene sequence

DFNB59 (deafness, autosomal recessive 59) (eg, autosomal recessive nonsyndromic hearing impairment), full gene sequence

DGUOK (deoxyguanosine kinase) (eg, hepatocerebral mitochondrial DNA depletion syndrome), full gene sequence

DHCR7 (7-dehydrocholesterol reductase) (eg, Smith-Lemli-Opitz syndrome), full gene sequence

EIF2B2 (eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa) (eg, leukoencephalopathy with vanishing white matter), full gene sequence

EMD (emerin) (eg, Emery-Dreifuss muscular dystrophy), full gene sequence

ENG (endoglin) (eg, hereditary hemorrhagic telangiectasia, type 1), duplication/deletion analysis

EYA1 (eyes absent homolog 1 [Drosophila]) (eg, branchio-oto-renal [BOR] spectrum disorders), duplication/deletion analysis

FGFR1 (fibroblast growth factor receptor 1) (eg, Kallmann syndrome 2), full gene sequence

FH (fumarate hydratase) (eg, fumarate hydratase deficiency, hereditary leiomyomatosis with renal cell cancer), full gene sequence

FKTN (fukutin) (eg, limb-girdle muscular dystrophy [LGMD] type 2M or 2L), full gene sequence

FTSJ1 (FtsJ RNA 2'-O-methyltransferase 1) (eg, X-linked intellectual disability 9), duplication/deletion analysis

GABRG2 (gamma-aminobutyric acid [GABA] A receptor, gamma 2) (eg, generalized epilepsy with febrile seizures), full gene sequence

GCH1 (GTP cyclohydrolase 1) (eg, autosomal dominant dopa-responsive dystonia), full gene sequence

GDAP1 (ganglioside-induced differentiation-associated protein 1) (eg, Charcot-Marie-Tooth disease), full gene sequence

GFAP (glial fibrillary acidic protein) (eg, Alexander disease), full gene sequence

GHR (growth hormone receptor) (eg, Laron syndrome), full gene sequence

GHRHR (growth hormone releasing hormone receptor) (eg, growth hormone deficiency), full gene sequence

GLA (galactosidase, alpha) (eg, Fabry disease), full gene sequence

HNF1A (HNF1 homeobox A) (eg, maturity-onset diabetes of the young [MODY]), full gene sequence

HNF1B (HNF1 homeobox B) (eg, maturity-onset diabetes of the young [MODY]), full gene sequence

HTRA1 (HtrA serine peptidase 1) (eg, macular degeneration), full gene sequence

IDS (iduronate 2-sulfatase) (eg, mucopolysacchridosis, type II), full gene sequence

IL2RG (interleukin 2 receptor, gamma) (eg, X-linked severe combined immunodeficiency), full gene sequence

ISPD (isoprenoid synthase domain containing) (eg, muscle-eye-brain disease, Walker-Warburg syndrome), full gene sequence

KRAS (Kirsten rat sarcoma viral oncogene homolog) (eg, Noonan syndrome), full gene sequence

LAMP2 (lysosomal-associated membrane protein 2) (eg, Danon disease), full gene sequence

LDLR (low density lipoprotein receptor) (eg, familial hypercholesterolemia), duplication/deletion analysis

MEN1 (multiple endocrine neoplasia I) (eg, multiple endocrine neoplasia type 1, Wermer syndrome), full gene sequence

MMAA (methylmalonic aciduria [cobalamine deficiency] type A) (eg, MMAA-related methylmalonic acidemia), full gene sequence

MMAB (methylmalonic aciduria [cobalamine deficiency] type B) (eg, MMAA-related methylmalonic acidemia), full gene sequence

MPI (mannose phosphate isomerase) (eg, congenital disorder of glycosylation 1b), full gene sequence

MPV17 (MpV17 mitochondrial inner membrane protein) (eg, mitochondrial DNA depletion syndrome), full gene sequence

MPZ (myelin protein zero) (eg, Charcot-Marie-Tooth), full gene sequence

MTM1 (myotubularin 1) (eg, X-linked centronuclear myopathy), duplication/deletion analysis

MYL2 (myosin, light chain 2, regulatory, cardiac, slow) (eg, familial hypertrophic cardiomyopathy), full gene sequence

MYL3 (myosin, light chain 3, alkali, ventricular, skeletal, slow) (eg, familial hypertrophic cardiomyopathy), full gene sequence

MYOT (myotilin) (eg, limb-girdle muscular dystrophy), full gene sequence

NDUFS7 (NADH dehydrogenase [ubiquinone] Fe-S protein 7, 20kDa [NADH-coenzyme Q reductase]) (eg, Leigh syndrome, mitochondrial complex I deficiency), full gene sequence

NDUFS8 (NADH dehydrogenase [ubiquinone] Fe-S protein 8, 23kDa [NADH-coenzyme Q reductase]) (eg, Leigh syndrome, mitochondrial complex I deficiency), full gene sequence

NDUFV1 (NADH dehydrogenase [ubiquinone] flavoprotein 1, 51kDa) (eg, Leigh syndrome, mitochondrial complex I deficiency), full gene sequence

NEFL (neurofilament, light polypeptide) (eg, Charcot-Marie-Tooth), full gene sequence

NF2 (neurofibromin 2 [merlin]) (eg, neurofibromatosis, type 2), duplication/deletion analysis

NLGN3 (neuroligin 3) (eg, autism spectrum disorders), full gene sequence

NLGN4X (neuroligin 4, X-linked) (eg, autism spectrum disorders), full gene sequence

NPHP1 (nephronophthisis 1 [juvenile]) (eg, Joubert syndrome), deletion analysis, and duplication analysis, if performed

NPHS2 (nephrosis 2, idiopathic, steroid-resistant [podocin]) (eg, steroid-resistant nephrotic syndrome), full gene sequence

NSD1 (nuclear receptor binding SET domain protein 1) (eg, Sotos syndrome), duplication/deletion analysis

OTC (ornithine carbamoyltransferase) (eg, ornithine transcarbamylase deficiency), full gene sequence

PAFAH1B1 (platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 [45kDa]) (eg, lissencephaly, Miller-Dieker syndrome), duplication/deletion analysis

PARK2 (Parkinson protein 2, E3 ubiquitin protein ligase [parkin]) (eg, Parkinson disease), duplication/deletion analysis

PCCA (propionyl CoA carboxylase, alpha polypeptide) (eg, propionic acidemia, type 1), duplication/deletion analysis

PCDH19 (protocadherin 19) (eg, epileptic encephalopathy), full gene sequence

PDHA1 (pyruvate dehydrogenase [lipoamide] alpha 1) (eg, lactic acidosis), duplication/deletion analysis

PDHB (pyruvate dehydrogenase [lipoamide] beta) (eg, lactic acidosis), full gene sequence

PINK1 (PTEN induced putative kinase 1) (eg, Parkinson disease), full gene sequence

PKLR (pyruvate kinase, liver and RBC) (eg, pyruvate kinase deficiency), full gene sequence

PLP1 (proteolipid protein 1) (eg, Pelizaeus-Merzbacher disease, spastic paraplegia), full gene sequence

POU1F1 (POU class 1 homeobox 1) (eg, combined pituitary hormone deficiency), full gene sequence

PRX (periaxin) (eg, Charcot-Marie-Tooth disease), full gene sequence

PQBP1 (polyglutamine binding protein 1) (eg, Renpenning syndrome), full gene sequence

PSEN1 (presenilin 1) (eg, Alzheimer disease), full gene sequence

RAB7A (RAB7A, member RAS oncogene family) (eg, Charcot-Marie-Tooth disease), full gene sequence

RAI1 (retinoic acid induced 1) (eg, Smith-Magenis syndrome), full gene sequence

REEP1 (receptor accessory protein 1) (eg, spastic paraplegia), full gene sequence

RET (ret proto-oncogene) (eg, multiple endocrine neoplasia, type 2A and familial medullary thyroid carcinoma), targeted sequence analysis (eg, exons 10, 11, 13-16)

RPS19 (ribosomal protein S19) (eg, Diamond-Blackfan anemia), full gene sequence

RRM2B (ribonucleotide reductase M2 B [TP53 inducible]) (eg, mitochondrial DNA depletion), full gene sequence

SCO1 (SCO cytochrome oxidase deficient homolog 1) (eg, mitochondrial respiratory chain complex IV deficiency), full gene sequence

SDHB (succinate dehydrogenase complex, subunit B, iron sulfur) (eg, hereditary paraganglioma), full gene sequence

SDHC (succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa) (eg, hereditary paraganglioma-pheochromocytoma syndrome), full gene sequence

SGCA (sarcoglycan, alpha [50kDa dystrophin-associated glycoprotein]) (eg, limb-girdle muscular dystrophy), full gene sequence

SGCB (sarcoglycan, beta [43kDa dystrophin-associated glycoprotein]) (eg, limb-girdle muscular dystrophy), full gene sequence

SGCD (sarcoglycan, delta [35kDa dystrophin-associated glycoprotein]) (eg, limb-girdle muscular dystrophy), full gene sequence

SGCE (sarcoglycan, epsilon) (eg, myoclonic dystonia), duplication/deletion analysis

SGCG (sarcoglycan, gamma [35kDa dystrophin-associated glycoprotein]) (eg, limb-girdle muscular dystrophy), full gene sequence

SHOC2 (soc-2 suppressor of clear homolog) (eg, Noonan-like syndrome with loose anagen hair), full gene sequence

SHOX (short stature homeobox) (eg, Langer mesomelic dysplasia), full gene sequence

SIL1 (SIL1 homolog, endoplasmic reticulum chaperone [S. cerevisiae]) (eg, ataxia), full gene sequence

SLC2A1 (solute carrier family 2 [facilitated glucose transporter], member 1) (eg, glucose transporter type 1 [GLUT 1] deficiency syndrome), full gene sequence

SLC16A2 (solute carrier family 16, member 2 [thyroid hormone transporter]) (eg, specific thyroid hormone cell transporter deficiency, Allan-Herndon-Dudley syndrome), full gene sequence

SLC22A5 (solute carrier family 22 [organic cation/carnitine transporter], member 5) (eg, systemic primary carnitine deficiency), full gene sequence

SLC25A20 (solute carrier family 25 [carnitine/acylcarnitine translocase], member 20) (eg, carnitine-acylcarnitine translocase deficiency), full gene sequence

SMAD4 (SMAD family member 4) (eg, hemorrhagic telangiectasia syndrome, juvenile polyposis), duplication/deletion analysis

SPAST (spastin) (eg, spastic paraplegia), duplication/deletion analysis

SPG7 (spastic paraplegia 7 [pure and complicated autosomal recessive]) (eg, spastic paraplegia), duplication/deletion analysis

SPRED1 (sprouty-related, EVH1 domain containing 1) (eg, Legius syndrome), full gene sequence

STAT3 (signal transducer and activator of transcription 3 [acute-phase response factor]) (eg, autosomal dominant hyper-IgE syndrome), targeted sequence analysis (eg, exons 12, 13, 14, 16, 17, 20, 21)

STK11 (serine/threonine kinase 11) (eg, Peutz-Jeghers syndrome), full gene sequence

SURF1 (surfeit 1) (eg, mitochondrial respiratory chain complex IV deficiency), full gene sequence

TARDBP (TAR DNA binding protein) (eg, amyotrophic lateral sclerosis), full gene sequence

TBX5 (T-box 5) (eg, Holt-Oram syndrome), full gene sequence

TCF4 (transcription factor 4) (eg, Pitt-Hopkins syndrome), duplication/deletion analysis

TGFBR1 (transforming growth factor, beta receptor 1) (eg, Marfan syndrome), full gene sequence

TGFBR2 (transforming growth factor, beta receptor 2) (eg, Marfan syndrome), full gene sequence

THRB (thyroid hormone receptor, beta) (eg, thyroid hormone resistance, thyroid hormone beta receptor deficiency), full gene sequence or targeted sequence analysis of >5 exons

TK2 (thymidine kinase 2, mitochondrial) (eg, mitochondrial DNA depletion syndrome), full gene sequence

TNNC1 (troponin C type 1 [slow]) (eg, hypertrophic cardiomyopathy or dilated cardiomyopathy), full gene sequence

TNNI3 (troponin I, type 3 [cardiac]) (eg, familial hypertrophic cardiomyopathy), full gene sequence

TPM1 (tropomyosin 1 [alpha]) (eg, familial hypertrophic cardiomyopathy), full gene sequence

TSC1 (tuberous sclerosis 1) (eg, tuberous sclerosis), duplication/deletion analysis

TYMP (thymidine phosphorylase) (eg, mitochondrial DNA depletion syndrome), full gene sequence

VWF (von Willebrand factor) (eg, von Willebrand disease type 2N), targeted sequence analysis (eg, exons 18-20, 23-25)

WT1 (Wilms tumor 1) (eg, Denys-Drash syndrome, familial Wilms tumor), full gene sequence

ZEB2 (zinc finger E-box binding homeobox 2) (eg, Mowat-Wilson syndrome), full gene sequence

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

Molecular pathology procedures are specialized tests conducted at the molecular level to diagnose, treat, and provide prognostic indicators for various genetic disorders, cancers, infectious diseases, and to assess tissue histocompatibility in transplant procedures. These procedures are categorized into different levels based on their complexity, which reflects the amount of professional work and laboratory costs involved in performing them. Specifically, Level 6 molecular pathology procedures encompass a range of analyses, including the examination of 6-10 exons through DNA sequence analysis, mutation scanning, or the identification of duplication/deletion variants across 11-25 exons. The process begins with a thorough review of the patient's medical history, clinical findings, and results from other diagnostic tests. Following this assessment, the Level 6 test is performed, which may include a variety of specific tests identified under CPT® Code 81405. It is important to note that molecular pathology procedures not explicitly listed but requiring similar levels of expertise, work, and laboratory costs should also be reported using this code. After the test is completed, the molecular pathologist interprets the results and generates a comprehensive written report detailing the findings, which is essential for guiding further clinical decisions.

© Copyright 2026 Coding Ahead. All rights reserved.

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