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Official Description

Fetal aneuploidy (trisomy 21, 18, and 13) DNA sequence analysis of selected regions using maternal plasma, algorithm reported as a risk score for each trisomy

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 81507 pertains to the non-invasive detection of fetal aneuploidy, specifically targeting trisomies 21, 18, and 13. This procedure involves the analysis of fetal DNA that is shed from the placenta and circulates within the maternal plasma. By utilizing advanced sequencing techniques, such as shotgun sequencing, multiplex ligation-dependent probe amplification (MLPA), and digital polymerase chain reaction (digital PCR), healthcare professionals can assess the risk of these chromosomal abnormalities. The algorithm generated from this analysis provides a risk score for each trisomy, offering a more accurate assessment compared to traditional methods that rely on maternal age and biochemical markers, including human chorionic gonadotropin (hCG), unconjugated estriol, alpha-fetoprotein, inhibin A, or pregnancy-associated plasma protein A (PAPP-A). While these conventional tests are often conducted initially, the results may lead to subsequent DNA sequencing for a more definitive risk evaluation. The implementation of non-invasive screening significantly reduces the necessity for more invasive diagnostic procedures, such as chorionic villus sampling and amniocentesis, which carry higher risks for both maternal and fetal complications.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure associated with CPT® Code 81507 is indicated for the non-invasive assessment of fetal aneuploidy, specifically for the following conditions:

  • Trisomy 21 This condition, also known as Down syndrome, is characterized by the presence of an extra chromosome 21 and is associated with developmental delays and physical disabilities.
  • Trisomy 18 Also known as Edwards syndrome, this condition involves an extra chromosome 18 and is associated with severe developmental and physical challenges, often leading to a significantly reduced lifespan.
  • Trisomy 13 Known as Patau syndrome, this condition results from an extra chromosome 13 and is associated with severe intellectual disability and physical abnormalities, often resulting in a short life expectancy.

2. Procedure

The procedure for CPT® Code 81507 involves several key steps to ensure accurate analysis of fetal DNA. First, a blood sample is collected from the pregnant individual, which contains maternal plasma. This plasma is then processed to isolate the fetal DNA that has been shed from the placenta. Following isolation, advanced sequencing techniques are employed to analyze the selected regions of the fetal DNA. Techniques such as shotgun sequencing, multiplex ligation-dependent probe amplification (MLPA), or digital polymerase chain reaction (digital PCR) may be utilized to perform the analysis. The results of this sequencing are then interpreted using an algorithm that calculates a risk score for each of the trisomies being assessed. This risk score provides a quantitative measure of the likelihood that the fetus may be affected by trisomy 21, 18, or 13, allowing healthcare providers to make informed decisions regarding further testing or monitoring.

3. Post-Procedure

After the completion of the fetal aneuploidy DNA sequence analysis, the results are typically communicated to the healthcare provider who ordered the test. The risk scores generated from the analysis can guide further clinical decision-making. If the risk scores indicate a higher likelihood of aneuploidy, the healthcare provider may discuss the option of more invasive diagnostic procedures, such as chorionic villus sampling or amniocentesis, which can provide definitive results. Additionally, the individual may be counseled regarding the implications of the results, including potential follow-up care and support resources. It is important to note that the non-invasive nature of this screening reduces the risk of complications associated with invasive procedures, making it a safer option for assessing fetal health.

Short Descr FETAL ANEUPLOIDY TRISOM RISK
Medium Descr FETAL ANEUPLOIDY 21 18 13 SEQ ANALY TRISOM RISK
Long Descr Fetal aneuploidy (trisomy 21, 18, and 13) DNA sequence analysis of selected regions using maternal plasma, algorithm reported as a risk score for each trisomy
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Service Paid under Fee Schedule or Payment System other than OPPS
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
Date
Action
Notes
2017-01-01 Changed Guideline added.
2014-01-01 Added Added
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