© Copyright 2026 American Medical Association. All rights reserved.
The CPT® Code 81514 pertains to a diagnostic test used to identify bacterial vaginosis and vaginitis through a quantitative real-time amplification of specific DNA markers. This test targets several key organisms associated with these conditions, including Gardnerella vaginalis, Atopobium vaginae, Megasphaera type 1, and Bacterial Vaginosis Associated Bacteria-2 (BVAB-2), as well as Lactobacillus species such as L. crispatus and L. jensenii. The procedure utilizes vaginal-fluid specimens to perform a nucleic acid amplification test (NAAT), which is a highly sensitive method for detecting the presence of these microorganisms. The results of the test are reported as either positive or negative, indicating a high likelihood of bacterial vaginosis. Additionally, the test includes the separate detection of Trichomonas vaginalis and various Candida species, such as C. albicans, C. tropicalis, C. parapsilosis, C. dubliniensis, Candida glabrata, and Candida krusei, when these are reported. This comprehensive approach allows for a detailed understanding of the microbial composition in the vaginal environment, which is crucial for diagnosing and managing conditions related to vaginitis.
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