CPT® Code 81541

Oncology (prostate), mRNA gene expression profiling by real-time RT-PCR of 46 genes (31 content and 15 housekeeping), utilizing formalin-fixed paraffin-embedded tissue, algorithm reported as a disease-specific mortality risk score

The CPT® Code 81541 pertains to a specific molecular genomic test utilized in the field of oncology, particularly for prostate cancer. This test employs real-time reverse transcription polymerase chain reaction (RT-PCR) to analyze the expression of messenger RNA (mRNA) from a total of 46 genes, which includes 31 content genes that are indicative of tumor behavior and 15 housekeeping genes that serve as controls to ensure the accuracy of the test results. The tissue sample used for this analysis is derived from formalin-fixed paraffin-embedded prostate tumor tissue, which is a standard method for preserving biological samples for histological examination. The primary focus of this test is to evaluate the expression levels of cell cycle progression (CCP) genes, which are critical in determining the likelihood of prostate cancer progression. The results of the test are reported as a disease-specific mortality risk score that ranges from -3 to +3. This score reflects the levels of CCP gene expression, with higher scores suggesting an increased risk of tumor growth or other adverse clinical outcomes. The insights gained from this genomic profiling can significantly influence clinical decision-making, particularly in formulating treatment strategies such as opting for active surveillance versus pursuing more aggressive surgical interventions.