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The CPT® Code 82261 refers to a laboratory test specifically designed to screen for biotinidase deficiency. Biotinidase is an enzyme that plays a crucial role in the metabolism of biotin, a vitamin that is essential for the proper processing of fats, proteins, and carbohydrates within the body. This enzyme is predominantly found in the liver, kidneys, and blood serum, where it functions to cleave biotin from biocytin, allowing the body to recycle this vital nutrient. Biotinidase deficiency is an inherited genetic disorder that significantly impairs the body's ability to reuse biotin, leading to a range of health issues. Symptoms of this deficiency typically manifest in infancy or early childhood and may include seizures, muscle weakness, decreased muscle tone (hypotonia), developmental delays, and respiratory problems. To conduct this test, a blood sample is collected, which can be obtained through a venipuncture or heel stick, both of which are separately reportable procedures. The serum or plasma from the blood sample is then analyzed using spectrophotometry, a method that measures the amount of light absorbed by the sample. It is important that the patient sample is paired with a control sample taken from a normal, healthy individual who is not biologically related to the patient, ensuring the accuracy and reliability of the test results.
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The biotinidase test (CPT® Code 82261) is indicated for the screening of biotinidase deficiency, which is an inherited genetic disorder. The following conditions and symptoms warrant the performance of this laboratory test:
The procedure for testing biotinidase levels involves several key steps that ensure accurate results. First, a blood sample is obtained from the patient. This can be done through a venipuncture, where a needle is inserted into a vein, or via a heel stick, which is a common method used for infants. The choice of method depends on the age and condition of the patient. Once the blood sample is collected, it is processed to separate the serum or plasma, which is the component that will be tested. The testing is performed using spectrophotometry, a technique that measures the concentration of biotinidase in the serum or plasma by analyzing the light absorption characteristics of the sample. To ensure the reliability of the test results, the patient sample must be paired with a control sample taken from a normal, healthy, non-biologically related individual. This control sample serves as a benchmark, allowing for accurate comparison and validation of the test results.
After the biotinidase test is completed, the patient may experience no significant post-procedure care requirements, especially if the blood sample was obtained through a heel stick or venipuncture, which are generally low-risk procedures. However, it is important to monitor the site of the blood draw for any signs of excessive bleeding or infection. The results of the test will typically be reviewed by a healthcare professional, who will discuss the findings with the patient and determine if any further action or treatment is necessary based on the results. If biotinidase deficiency is confirmed, appropriate management and treatment options will be explored to address the condition and mitigate any associated symptoms.
| Short Descr | ASSAY OF BIOTINIDASE | Medium Descr | BIOTINIDASE EACH SPECIMEN | Long Descr | Biotinidase, each specimen | Status Code | Statutory Exclusion (from MPFS, may be paid under other methodologies) | Global Days | XXX - Global Concept Does Not Apply | PC/TC Indicator (26, TC) | 9 - Not Applicable | Multiple Procedures (51) | 9 - Concept does not apply. | Bilateral Surgery (50) | 9 - Concept does not apply. | Physician Supervisions | 09 - Concept does not apply. | Assistant Surgeon (80, 82) | 9 - Concept does not apply. | Co-Surgeons (62) | 9 - Concept does not apply. | Team Surgery (66) | 9 - Concept does not apply. | Diagnostic Imaging Family | 99 - Concept Does Not Apply | CLIA Waived (QW) | No | APC Status Indicator | Conditionally packaged laboratory tests | Type of Service (TOS) | 5 - Diagnostic Laboratory | Berenson-Eggers TOS (BETOS) | T1H - Lab tests - other (non-Medicare fee schedule) | MUE | 1 | CCS Clinical Classification | 233 - Laboratory - Chemistry and Hematology |
| 90 | Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number. | GA | Waiver of liability statement issued as required by payer policy, individual case | Q4 | Service for ordering/referring physician qualifies as a service exemption |
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| 1999-01-01 | Added | First appearance in code book in 1999. |
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