Coding Ahead
CasePilot
Medical Coding Assistant
Case2Code
Search and Code Lookup Tool
CareerCenter
Medical Coding Job Board
Log in Register free account

Need help choosing the right code?

Ask CasePilot about procedures, modifiers, bundling, and coding guidance.

Try CasePilot

Official Description

Column chromatography, includes mass spectrometry, if performed (eg, HPLC, LC, LC/MS, LC/MS-MS, GC, GC/MS-MS, GC/MS, HPLC/MS), non-drug analyte(s) not elsewhere specified, qualitative or quantitative, each specimen

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

Column chromatography, often combined with mass spectrometry, is a sophisticated analytical technique used to separate and identify chemical components within a sample. This method involves two phases: a stationary phase, where the sample is placed, and a mobile phase, which can be either a gas or liquid that moves through the stationary phase. As the sample is introduced, the various components interact differently with the stationary phase, leading to their separation. Once separated, these components are then measured and analyzed using mass spectrometry, which provides precise information about their molecular structure and weight. This dual approach enhances the sensitivity and specificity of the analysis, making it particularly effective for detecting a wide range of substances, including contaminants in biological fluids such as blood, urine, oral fluids, hair, and sweat. The CPT® Code 82542 encompasses both qualitative and quantitative assessments of non-drug analytes that are not specified elsewhere, ensuring comprehensive testing for each specimen analyzed.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure coded under CPT® 82542 is indicated for the analysis of non-drug analyte(s) that are not specified elsewhere. This testing is essential in various clinical and research settings where the identification and quantification of chemical substances are necessary. The following conditions may warrant the use of this procedure:

  • Detection of Chemical Contaminants This procedure is utilized to identify and quantify chemical contaminants present in biological samples such as blood, urine, oral fluids, hair, and sweat.
  • Analysis of Metabolites It is indicated for the analysis of metabolites that may be present in a patient's biological samples, aiding in the understanding of metabolic processes.
  • Research Applications This method is often employed in research settings to analyze complex mixtures of substances, providing insights into chemical interactions and compositions.

2. Procedure

The procedure for CPT® 82542 involves several key steps that ensure accurate separation and analysis of the chemical components within a specimen. The following outlines the procedural steps:

  • Step 1: Sample Preparation The initial step involves preparing the specimen, which may include blood, urine, or other biological fluids. This preparation is crucial to ensure that the sample is suitable for analysis and free from contaminants that could interfere with the results.
  • Step 2: Chromatography Setup The prepared sample is then introduced into the chromatography system. Depending on the specific method used, such as liquid chromatography (LC) or gas chromatography (GC), the sample is subjected to a stationary phase where separation occurs based on the chemical properties of the components.
  • Step 3: Separation of Components As the mobile phase moves through the stationary phase, the various components of the sample are separated based on their interactions with the stationary phase. This separation allows for the isolation of individual analytes for further analysis.
  • Step 4: Mass Spectrometry Analysis Following separation, the isolated components are directed into the mass spectrometer. Here, they are ionized and measured, providing detailed information about their molecular weight and structure. This step is critical for identifying the specific chemical compounds present in the sample.
  • Step 5: Data Interpretation The final step involves interpreting the data obtained from the mass spectrometry analysis. This interpretation allows for qualitative or quantitative assessment of the non-drug analyte(s) present in the specimen, leading to informed clinical decisions or research conclusions.

3. Post-Procedure

After the completion of the column chromatography and mass spectrometry analysis, the results are compiled and reviewed. It is essential to ensure that the data is accurately interpreted and documented. Depending on the findings, further clinical action may be required, such as additional testing or treatment recommendations. The procedure does not typically require extensive post-procedure care, but it is important for healthcare professionals to communicate the results to the patient or referring physician, ensuring that any necessary follow-up actions are taken based on the analysis outcomes.

Short Descr COL CHROMOTOGRAPHY QUAL/QUAN
Medium Descr COL-CHR/MS NONDRUG ANALYTE NES QUAL/QUAN EA SPEC
Long Descr Column chromatography, includes mass spectrometry, if performed (eg, HPLC, LC, LC/MS, LC/MS-MS, GC, GC/MS-MS, GC/MS, HPLC/MS), non-drug analyte(s) not elsewhere specified, qualitative or quantitative, each specimen
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 6
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
76 Repeat procedure or service by same physician or other qualified health care professional: it may be necessary to indicate that a procedure or service was repeated by the same physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 76 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
GA Waiver of liability statement issued as required by payer policy, individual case
Q3 Live kidney donor surgery and related services
GW Service not related to the hospice patient's terminal condition
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
GZ Item or service expected to be denied as not reasonable and necessary
GC This service has been performed in part by a resident under the direction of a teaching physician
GX Notice of liability issued, voluntary under payer policy
Q4 Service for ordering/referring physician qualifies as a service exemption
TC Technical component; under certain circumstances, a charge may be made for the technical component alone; under those circumstances the technical component charge is identified by adding modifier 'tc' to the usual procedure number; technical component charges are institutional charges and not billed separately by physicians; however, portable x-ray suppliers only bill for technical component and should utilize modifier tc; the charge data from portable x-ray suppliers will then be used to build customary and prevailing profiles
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
Date
Action
Notes
2016-01-01 Changed Description Changed
2015-01-01 Changed Description Changed
2011-01-01 Changed Short description changed.
2001-01-01 Changed Code description changed.
1999-01-01 Added First appearance in code book in 1999.
Code
Description
Code
Description
Code
Description
Code
Description
CasePilot

Get instant expert-level medical coding assistance.

Ask about:
CPT Codes Guidelines Modifiers Crosswalks NCCI Edits Compliance Medicare Coverage
Example: "What is CPT code 99213?" or "Guidelines for E/M services"