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CPT®
82543

CPT® Code 82543
Deleted

Code deleted, to report see analyte code or 82542

Official Description

Column chromatography/mass spectrometry (eg, GC/MS, or HPLC/MS), non-drug analyte not elsewhere specified; stable isotope dilution, single analyte, quantitative, single stationary and mobile phase

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

Column chromatography/mass spectrometry is a sophisticated analytical technique that combines the principles of chromatography and mass spectrometry to analyze chemical substances. In this method, chromatography is employed to separate various chemical components based on their different affinities to a stationary phase and a mobile phase, which can be either gas or liquid. Once the components are separated, mass spectrometry is utilized to capture and measure the isolated molecules as they elute from the chromatography column. This process allows for the precise determination of the chemical structure of the components being analyzed. The addition of stable isotope dilution enhances the sensitivity and selectivity of the technique, enabling the quantification of a wider range of chemical compounds. In this context, a stable isotope of the analyte is introduced into the sample, resulting in an isotopically labeled "spike" that facilitates accurate measurement during the gas chromatography/mass spectrometry (GC/MS) or high-performance liquid chromatography/mass spectrometry (HPLC/MS) processes. The CPT® code 82543 is specifically designated for the quantification of a single non-drug analyte that does not have a specified code, while the code 82544 is used when quantifying multiple analytes, such as acylglycines and essential fatty acids.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure of column chromatography/mass spectrometry (CPT® Code 82543) is indicated for the quantitative analysis of specific non-drug analytes that are not classified under any other specified code. This method is particularly useful in various clinical and research settings where precise measurement of chemical compounds is necessary.

  • Non-drug analytes This procedure is performed to quantify single non-drug analytes that lack a designated CPT® code, allowing for accurate assessment of these substances.

2. Procedure

The procedure involves several critical steps to ensure accurate separation and quantification of the analytes.

  • Step 1: Sample Preparation The initial step involves preparing the sample by adding a stable isotope of the analyte to be measured. This stable isotope acts as a reference point during the analysis, allowing for more precise quantification.
  • Step 2: Chromatographic Separation The prepared sample is then subjected to column chromatography, where it is introduced into a chromatography column. The stationary phase within the column interacts with the components of the sample, while the mobile phase, which can be either gas or liquid, facilitates the movement of these components through the column. As the sample progresses through the column, different components are separated based on their chemical properties.
  • Step 3: Mass Spectrometry Analysis Following the chromatographic separation, the isolated components elute from the column and enter the mass spectrometer. Here, the mass spectrometer measures the mass-to-charge ratio of the ions produced from the analytes. This data is crucial for determining the chemical structure and quantity of the analytes present in the sample.
  • Step 4: Data Interpretation The final step involves analyzing the data obtained from the mass spectrometry. The results are interpreted to quantify the concentration of the non-drug analyte in the sample, providing valuable information for clinical or research purposes.

3. Post-Procedure

After the completion of the column chromatography/mass spectrometry procedure, it is essential to ensure proper handling and storage of the data obtained. The results should be documented accurately, and any necessary follow-up actions based on the findings should be determined. Additionally, the stability of the analytes should be considered, as some may require specific storage conditions to maintain their integrity for future analysis. Overall, the procedure is designed to provide reliable and precise quantification of non-drug analytes, contributing to effective clinical decision-making and research outcomes.
Short Descr COLUMN CHROMOTOGRAPH/ISOTOPE
Medium Descr COL-CHR/MS STABLE ISOTOPE DIL 1 ANALYTE NES
Long Descr Column chromatography/mass spectrometry (eg, GC/MS, or HPLC/MS), non-drug analyte not elsewhere specified; stable isotope dilution, single analyte, quantitative, single stationary and mobile phase
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
APC Status Indicator Items and Services Packaged into APC Rates
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE Not applicable/unspecified.
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
Date
Action
Notes
2016-01-01 Deleted Code deleted, to report see analyte code or 82542
2015-01-01 Changed Description Changed
2001-01-01 Changed Code description changed.
1999-01-01 Added First appearance in code book in 1999.
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