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Official Description

Vitamin D; 1, 25 dihydroxy, includes fraction(s), if performed

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 82652 refers to the laboratory test for measuring blood levels of 1, 25-dihydroxyvitamin D, which is a biologically active form of vitamin D. This test is particularly significant in clinical settings where there is a suspicion of elevated calcium levels in the blood, as it aids in diagnosing conditions that may lead to abnormal vitamin D metabolism and calcium regulation. Such conditions include diseases like sarcoidosis and lymphoma, which can cause the body to produce excess vitamin D. Additionally, the measurement of 1, 25-dihydroxyvitamin D is crucial for evaluating the function of the parathyroid glands, which play a vital role in calcium homeostasis. The procedure involves obtaining a blood sample from the patient, and the analysis is typically performed using a method known as radioimmunoassay. The results of this test may provide a comprehensive view of the patient's vitamin D status, reflecting both the total levels of 1, 25-dihydroxyvitamin D and any fractions that may be present, thereby offering valuable insights for diagnosis and monitoring of related health issues.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

High Calcium Levels The measurement of 1, 25-dihydroxyvitamin D is indicated when a patient presents with elevated calcium levels in the blood, which may suggest underlying health issues.

Sarcoidosis This test is performed to determine if a patient has sarcoidosis, a condition that can lead to increased production of vitamin D.

Lymphoma The evaluation of 1, 25-dihydroxyvitamin D levels is also indicated in cases of lymphoma, as this malignancy can affect vitamin D metabolism.

Parathyroid Gland Disease The test may be utilized to diagnose or monitor diseases affecting the parathyroid glands, which are crucial for regulating calcium levels in the body.

2. Procedure

Step 1: Blood Sample Collection The procedure begins with the collection of a blood sample from the patient. This is typically done through venipuncture, where a needle is inserted into a vein, usually in the arm, to draw the necessary volume of blood for testing.

Step 2: Laboratory Analysis Once the blood sample is obtained, it is sent to a laboratory for analysis. The laboratory utilizes a technique known as radioimmunoassay to measure the levels of 1, 25-dihydroxyvitamin D in the sample. This method involves the use of radioactively labeled antibodies that specifically bind to the vitamin D metabolite, allowing for accurate quantification.

Step 3: Result Interpretation After the analysis is complete, the laboratory generates a report detailing the levels of 1, 25-dihydroxyvitamin D present in the blood sample. The results may include both the total amount of 1, 25-dihydroxyvitamin D and any fractions that represent this metabolite, providing comprehensive information for the healthcare provider.

3. Post-Procedure

Post-procedure care for the patient is generally minimal, as the blood draw is a routine procedure with low risk. Patients may be advised to drink fluids to help replenish any lost volume. The results of the test will typically be reviewed by the healthcare provider, who will discuss the findings with the patient and determine any necessary follow-up actions based on the levels of 1, 25-dihydroxyvitamin D and the clinical context. It is important for patients to understand that further testing or treatment may be required depending on the results and their overall health status.

Short Descr VIT D 1 25-DIHYDROXY
Medium Descr 1 25 DIHYDROXY INCLUDES FRACTIONS IF PERFORMED
Long Descr Vitamin D; 1, 25 dihydroxy, includes fraction(s), if performed
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
GA Waiver of liability statement issued as required by payer policy, individual case
GZ Item or service expected to be denied as not reasonable and necessary
GW Service not related to the hospice patient's terminal condition
GV Attending physician not employed or paid under arrangement by the patient's hospice provider
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
76 Repeat procedure or service by same physician or other qualified health care professional: it may be necessary to indicate that a procedure or service was repeated by the same physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 76 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
77 Repeat procedure by another physician or other qualified health care professional: it may be necessary to indicate that a basic procedure or service was repeated by another physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 77 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
AY Item or service furnished to an esrd patient that is not for the treatment of esrd
CC Procedure code change (use 'cc' when the procedure code submitted was changed either for administrative reasons or because an incorrect code was filed)
GC This service has been performed in part by a resident under the direction of a teaching physician
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
Q1 Routine clinical service provided in a clinical research study that is in an approved clinical research study
Q3 Live kidney donor surgery and related services
Date
Action
Notes
2011-01-01 Changed Short description changed.
2010-01-01 Changed Code description changed.
2009-01-01 Changed Code description changed
Pre-1990 Added Code added.
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