CPT® Code 83914

Code deleted, see 81200-81479

Official Description

Mutation identification by enzymatic ligation or primer extension, single segment, each segment (eg, oligonucleotide ligation assay [OLA], single base chain extension [SBCE], or allele-specific primer extension [ASPE])

Common Language Description

The CPT® Code 83914 refers to a specialized laboratory procedure used in molecular genetics to identify mutations or abnormalities within a specific segment of DNA. This process involves the analysis of a molecular sequence, which is a precise arrangement of nucleotides that make up the genetic material. The primary goal of this procedure is to detect mutations that may be associated with various genetic disorders or conditions. The techniques utilized in this code include enzymatic ligation or primer extension methods, which are critical for accurately pinpointing the location of the mutation. Examples of these techniques include the oligonucleotide ligation assay (OLA), single base chain extension (SBCE), and allele-specific primer extension (ASPE). Each of these methods allows for the examination of a single segment of DNA, providing valuable information for genetic testing and research. This procedure is essential for clinicians and researchers who require detailed insights into genetic variations that may impact patient care or contribute to the understanding of hereditary diseases.

1. Indications

The procedure associated with CPT® Code 83914 is indicated for the identification of specific mutations or abnormalities in genetic material. This analysis is crucial in various clinical and research settings, particularly for the following conditions:

Genetic Disorders Identification of mutations that may lead to hereditary conditions, allowing for better diagnosis and management.
Oncology Detection of mutations in cancer-related genes, which can inform treatment decisions and prognostic evaluations.
Pharmacogenomics Assessment of genetic variations that affect drug metabolism and response, aiding in personalized medicine approaches.

2. Procedure

The procedure for CPT® Code 83914 involves several key steps to ensure accurate mutation identification. Each step is critical for the successful analysis of the DNA segment.

Step 1: Sample Preparation The first step involves obtaining a sample of the patient's DNA, which can be extracted from various biological materials such as blood, saliva, or tissue. The quality and integrity of the DNA are assessed to ensure that it is suitable for further analysis.
Step 2: Enzymatic Ligation or Primer Extension In this step, specific techniques such as oligonucleotide ligation assay (OLA), single base chain extension (SBCE), or allele-specific primer extension (ASPE) are employed. These methods utilize enzymes to either ligate DNA fragments or extend primers that are complementary to the target mutation. This allows for the amplification of the specific segment of interest.
Step 3: Mutation Detection Following the enzymatic processes, the amplified segments are analyzed to identify the presence of mutations. This may involve various detection methods, including gel electrophoresis or real-time PCR, to visualize and confirm the results.

3. Post-Procedure

After the completion of the procedure associated with CPT® Code 83914, the results are compiled and interpreted by laboratory professionals. The findings are then communicated to the requesting physician, who will use the information to guide clinical decisions. It is important to note that the interpretation of the results may require additional context regarding the patient's clinical history and other diagnostic tests. Patients may also need counseling regarding the implications of the findings, especially if a genetic mutation is identified that could affect their health or that of their family members. Follow-up testing or additional genetic counseling may be recommended based on the results obtained.

Short Descr	MUTATION IDENT OLA/SBCE/ASPE
Medium Descr	MUTATION ID ENZYMATIC LIG/PRIMER XTN 1 SGM EA
Long Descr	Mutation identification by enzymatic ligation or primer extension, single segment, each segment (eg, oligonucleotide ligation assay [OLA], single base chain extension [SBCE], or allele-specific primer extension [ASPE])
Status Code	Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days	XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC)	9 - Not Applicable
Multiple Procedures (51)	9 - Concept does not apply.
Bilateral Surgery (50)	9 - Concept does not apply.
Physician Supervisions	09 - Concept does not apply.
Assistant Surgeon (80, 82)	9 - Concept does not apply.
Co-Surgeons (62)	9 - Concept does not apply.
Team Surgery (66)	9 - Concept does not apply.
Diagnostic Imaging Family	99 - Concept Does Not Apply
APC Status Indicator	Discontinued Code
Type of Service (TOS)	5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS)	none
MUE	Not applicable/unspecified.
CCS Clinical Classification	233 - Laboratory - Chemistry and Hematology