CPT® Code 85130

The chromogenic substrate assay, identified by CPT® Code 85130, is a specialized analytical technique utilized to assess enzyme activity within biological samples. This method involves the addition of a chromogenic-linked substrate to a sample of body tissue or fluid, resulting in a color change that correlates with the activity level of the specific enzyme or factor being measured. The intensity of the color produced serves as an indicator of the relative activity of the target factor, allowing for quantitative analysis. This assay is particularly valuable in the context of evaluating the effects of anti-thrombotic medications, such as heparin, as well as in the assessment of anticoagulant proteins like antithrombin (AT) and Protein C (PC). Additionally, it plays a role in the analysis of various components of the fibrinolytic system. To perform this assay, a blood sample is required, which is obtained through a separately reportable venipuncture procedure. Furthermore, the assay can be employed to quantitatively test plasma for heparin cofactor II, enhancing its utility in clinical settings where monitoring of coagulation factors is essential.