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Official Description

Fluorescent noninfectious agent antibody; screen, each antibody

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 86255 refers to the screening for antibodies to noninfectious agents using a fluorescent technique. This procedure is primarily utilized to detect the presence of specific antibodies in a patient's blood sample or cerebral spinal fluid (CSF). The screening is typically the first step in the diagnostic process, allowing healthcare providers to identify potential autoimmune disorders or other conditions that are not caused by infectious agents. If the screening indicates the presence of antibodies, a subsequent titer count may be performed using CPT® Code 86256 to quantify the level of these antibodies. The antibodies detected through this screening can be associated with various medical conditions, including celiac disease, premature ovarian failure, myasthenia gravis, and other autoimmune disorders. Additionally, this test can help assess exposure to environmental toxins that may lead to autoimmune diseases, as well as monitor conditions such as cancers, graft versus host disease following organ transplants, and myocardial infarction or other cardiac injuries. Furthermore, these antibody screenings and titers can serve as valuable markers for evaluating the effectiveness of immunosuppressant therapy in patients undergoing treatment for autoimmune conditions.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The screening for antibodies to noninfectious agents using CPT® Code 86255 is indicated for various medical conditions and situations, including:

  • Celiac Disease - A condition where the ingestion of gluten leads to damage in the small intestine, often requiring antibody testing for diagnosis.
  • Premature Ovarian Failure - A condition characterized by the loss of normal ovarian function before age 40, which may involve autoimmune factors.
  • Myasthenia Gravis - An autoimmune disorder that affects communication between nerves and muscles, leading to weakness and fatigue.
  • Other Autoimmune Disorders - Various conditions where the immune system mistakenly attacks the body’s own tissues, necessitating antibody screening.
  • Exposure to Environmental Toxins - Situations where individuals may have been exposed to substances that could trigger autoimmune responses.
  • Cancers - Certain malignancies may be associated with specific antibody responses, warranting screening.
  • Graft vs. Host Disease - A complication following organ transplants where the donor's immune cells attack the recipient's body.
  • Myocardial Infarction and Other Cardiac Injury - Conditions affecting the heart that may be linked to autoimmune processes.

2. Procedure

The procedure for screening antibodies to noninfectious agents involves several key steps, which are detailed as follows:

  • Step 1: Sample Collection - A blood sample or cerebral spinal fluid (CSF) is obtained from the patient. Blood is typically collected through a venipuncture, while CSF is obtained via a lumbar puncture, also known as a spinal tap. These methods ensure that the necessary biological material is available for testing.
  • Step 2: Laboratory Analysis - The collected sample is then subjected to a fluorescent technique in the laboratory. This method involves using fluorescent dyes that bind to specific antibodies present in the sample, allowing for the detection of these antibodies when exposed to light.
  • Step 3: Interpretation of Results - After the analysis, the laboratory interprets the results to determine the presence and levels of antibodies against noninfectious agents. If antibodies are detected, further testing may be warranted to quantify the antibody levels using a titer count.

3. Post-Procedure

Post-procedure care for patients undergoing antibody screening typically involves monitoring for any immediate adverse reactions, especially if a lumbar puncture was performed. Patients may experience mild discomfort or headache following the procedure. It is important for healthcare providers to inform patients about potential symptoms and when to seek further medical attention. Additionally, the results of the antibody screening should be discussed with the patient, including any necessary follow-up tests or treatments based on the findings. If antibodies are present, further evaluation may be required to assess the underlying condition and determine appropriate management strategies.

Short Descr FLUORESCENT ANTIBODY SCREEN
Medium Descr FLUORESCENT NONNFCT AGT ANTB SCREEN EA ANTIBODY
Long Descr Fluorescent noninfectious agent antibody; screen, each antibody
Status Code Active Code
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 6 - Laboratory Physician Interpretation Code
Multiple Procedures (51) 0 - No payment adjustment rules for multiple procedures apply.
Bilateral Surgery (50) 0 - 150% payment adjustment for bilateral procedures does NOT apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 0 - Payment restriction for assistants at surgery applies to this procedure...
Co-Surgeons (62) 0 - Co-surgeons not permitted for this procedure.
Team Surgery (66) 0 - Team surgeons not permitted for this procedure.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 5
CCS Clinical Classification 235 - Other Laboratory
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
Q4 Service for ordering/referring physician qualifies as a service exemption
GC This service has been performed in part by a resident under the direction of a teaching physician
GA Waiver of liability statement issued as required by payer policy, individual case
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
GZ Item or service expected to be denied as not reasonable and necessary
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
77 Repeat procedure by another physician or other qualified health care professional: it may be necessary to indicate that a basic procedure or service was repeated by another physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 77 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
GW Service not related to the hospice patient's terminal condition
76 Repeat procedure or service by same physician or other qualified health care professional: it may be necessary to indicate that a procedure or service was repeated by the same physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 76 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
AQ Physician providing a service in an unlisted health professional shortage area (hpsa)
AY Item or service furnished to an esrd patient that is not for the treatment of esrd
G8 Monitored anesthesia care (mac) for deep complex, complicated, or markedly invasive surgical procedure
PO Excepted service provided at an off-campus, outpatient, provider-based department of a hospital
Q1 Routine clinical service provided in a clinical research study that is in an approved clinical research study
TC Technical component; under certain circumstances, a charge may be made for the technical component alone; under those circumstances the technical component charge is identified by adding modifier 'tc' to the usual procedure number; technical component charges are institutional charges and not billed separately by physicians; however, portable x-ray suppliers only bill for technical component and should utilize modifier tc; the charge data from portable x-ray suppliers will then be used to build customary and prevailing profiles
X5 Diagnostic services requested by another clinician: for reporting services by a clinician who furnishes care to the patient only as requested by another clinician or subsequent and related services requested by another clinician; this modifier is reported for patient relationships that may not be adequately captured by the above alternative categories; reporting clinician service examples include but are not limited to, the radiologist's interpretation of an imaging study requested by another clinician
XE Separate encounter, a service that is distinct because it occurred during a separate encounter
XS Separate structure, a service that is distinct because it was performed on a separate organ/structure
Date
Action
Notes
2011-01-01 Changed Short description changed.
Pre-1990 Added Code added.
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