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Official Description

Immunoassay for tumor antigen, other antigen, quantitative (eg, CA 50, 72-4, 549), each

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

A quantitative immunoassay for tumor antigen, coded as CPT® 86316, is a laboratory test that measures the levels of specific tumor antigens in the serum. Tumor antigens, also known as tumor markers, are substances produced by cancer cells or by the body in response to cancer. The presence of significant levels of these antigens can indicate the presence of malignancy. This particular code encompasses a range of tumor antigens that are not specified elsewhere in the coding system. Examples of tumor antigens associated with this code include CA 50, CA 72-4, and CA 549. Each of these antigens is linked to specific types of cancers; for instance, CA 50 is primarily associated with gastrointestinal cancers such as pancreatic, gastric, colorectal, and hepatic carcinomas. CA 72-4 is utilized in the detection of gastrointestinal and breast cancers, while CA 549 is specifically relevant for breast cancer patients. Various immunoassay techniques are employed to detect these tumor antigens, including the use of monoclonal antibodies and radioimmunoassay for CA 50, CA 72-4, and CA 549. Additionally, soluble mesothelin-related peptides, another tumor antigen tested under this code, are evaluated using enzyme-linked immunosorbent assay (ELISA) to assist in the management of mesothelioma. Other methods such as enzyme immunoassay and chemiluminescent immunoassay may also be utilized for different tumor antigens. Regular testing of tumor antigens is an important part of cancer management, as it helps in monitoring the progression or regression of the disease in conjunction with other clinical assessments.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The quantitative immunoassay for tumor antigen, CPT® 86316, is indicated for the following conditions:

  • CA 50 Used to monitor gastrointestinal cancers, including pancreatic, gastric, colorectal, and hepatic carcinomas.
  • CA 72-4 Utilized in the detection and monitoring of gastrointestinal and breast cancers.
  • CA 549 Specifically relevant for patients diagnosed with breast cancer.
  • Soluble mesothelin-related peptides Tested to assist in the management of mesothelioma.

2. Procedure

The procedure for conducting a quantitative immunoassay for tumor antigen involves several key steps:

  • Sample Collection A blood sample is drawn from the patient, typically from a vein in the arm. This sample is then processed to separate the serum, which contains the tumor antigens to be measured.
  • Assay Technique The serum is subjected to a specific immunoassay technique. For tumor antigens such as CA 50, CA 72-4, and CA 549, monoclonal antibodies are used in conjunction with a radioimmunoassay method to detect and quantify the levels of these antigens. For soluble mesothelin-related peptides, an enzyme-linked immunosorbent assay (ELISA) is employed.
  • Data Analysis After the assay is completed, the results are analyzed to determine the concentration of the tumor antigens in the serum. This quantitative data is crucial for assessing the presence of malignancy and monitoring treatment efficacy.
  • Reporting Results The findings are compiled into a report that details the levels of the tumor antigens tested. This report is then provided to the healthcare provider for interpretation and further clinical decision-making.

3. Post-Procedure

Post-procedure care for patients undergoing a quantitative immunoassay for tumor antigen typically involves monitoring for any immediate reactions to the blood draw, although such reactions are rare. Patients may be advised to follow up with their healthcare provider to discuss the results of the test and any necessary next steps in their treatment or monitoring plan. Regular monitoring of tumor antigen levels is essential for evaluating the effectiveness of ongoing treatment and for making informed decisions regarding patient care.

Short Descr IMMUNOASSAY TUMOR OTHER
Medium Descr IMMUNOASSAY TUMOR ANTIGEN QUANTITATIVE
Long Descr Immunoassay for tumor antigen, other antigen, quantitative (eg, CA 50, 72-4, 549), each
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 2
CCS Clinical Classification 235 - Other Laboratory
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
GZ Item or service expected to be denied as not reasonable and necessary
GA Waiver of liability statement issued as required by payer policy, individual case
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
Q4 Service for ordering/referring physician qualifies as a service exemption
GV Attending physician not employed or paid under arrangement by the patient's hospice provider
GW Service not related to the hospice patient's terminal condition
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
PD Diagnostic or related non diagnostic item or service provided in a wholly owned or operated entity to a patient who is admitted as an inpatient within 3 days
XP Separate practitioner, a service that is distinct because it was performed by a different practitioner
XS Separate structure, a service that is distinct because it was performed on a separate organ/structure
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
Date
Action
Notes
2011-01-01 Changed Short description changed.
2005-01-01 Changed Code description changed.
2001-01-01 Changed Code description changed.
Pre-1990 Added Code added.
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