CPT® Code 86327

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Immunoelectrophoresis, crossed or two-dimensional, is a sophisticated laboratory technique that combines two distinct methods: conventional electrophoretic separation and electroimmunodiffusion. In the first phase of the procedure, electrophoresis is conducted to separate proteins based on their size and charge. Following this initial separation, the protein strip is placed onto a slide that contains an agarose solution infused with specific antibodies. This agarose solution is allowed to solidify, creating a stable medium for the next step. The second phase involves performing electrophoresis again, but this time at a right angle to the original separation. This two-dimensional approach allows for a more detailed analysis of the proteins, as it enables the identification and characterization of various protein components within a sample. The technique is particularly useful in diagnosing and monitoring certain diseases by providing insights into the protein composition of biological fluids.