CPT® Code 86356

A quantitative mononuclear cell antigen test, represented by CPT® Code 86356, is a laboratory procedure that utilizes flow cytometry to measure specific antigens present on mononuclear cells. Mononuclear cells, which include lymphocytes and monocytes, are isolated from heparinized whole blood, ensuring that the blood sample remains in a liquid state for accurate analysis. The process begins with the harvesting of these cells, followed by dilution of the mononuclear fraction to prepare it for testing. Paired aliquots of the diluted sample are then incubated with monoclonal antibodies that specifically bind to the target antigens. This binding allows for the identification and quantification of the antigens present in the sample. After incubation, the samples, along with control specimens, are analyzed using flow cytometry, a sophisticated technology that enables the rapid measurement of multiple parameters of individual cells as they flow in a stream through a laser beam. It is important to note that this code is applicable for each antigen tested, making it essential for accurate reporting and billing in laboratory settings where multiple antigens may be assessed in a single testing session.