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Official Description

Neutralization test, viral

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 86382 refers to a laboratory procedure known as a neutralization test for viruses. This test is specifically designed to measure the presence of neutralizing antibodies in a patient's blood, which are critical for determining immunity against certain viral infections, such as polio or rabies. Neutralizing antibodies are proteins produced by the immune system that can bind to viruses, preventing them from infecting cells and thereby neutralizing their harmful effects. The presence of these antibodies indicates that the individual may have developed immunity to the virus in question. One common application of this test is the rapid fluorescent focus inhibition test (RFFIT), which is particularly utilized for rabies testing. The RFFIT is typically conducted 14 to 21 days after a patient has completed an initial series of rabies vaccinations or after they have received a booster dose. For individuals who are at continuous risk of rabies exposure, such as those working with animals or in certain healthcare settings, this test may be performed every six months. Conversely, individuals with less frequent exposure may undergo testing every two years. The procedure involves obtaining a blood sample through venipuncture, which is a separately reportable procedure, and then analyzing the sample using culture and neutralization techniques to assess the presence and functionality of the antibodies against the live virus.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The neutralization test, coded as CPT® 86382, is indicated for the following conditions:

  • Assessment of Immunity This test is performed to evaluate the presence of neutralizing antibodies against specific viruses, such as polio or rabies, which may indicate immunity.
  • Post-Vaccination Evaluation The test is particularly relevant for individuals who have completed a rabies vaccination series, as it helps determine the effectiveness of the vaccine in eliciting an immune response.
  • Risk Assessment for Rabies Exposure Individuals who are at continuous risk for rabies exposure, such as those in veterinary or laboratory settings, may require this test to monitor their antibody levels regularly.

2. Procedure

The procedure for conducting the neutralization test involves several key steps:

  • Step 1: Patient Preparation Prior to the test, the patient may be informed about the procedure and any necessary preparations, such as fasting or avoiding certain medications, although specific instructions may vary based on clinical guidelines.
  • Step 2: Blood Sample Collection A blood sample is obtained from the patient through a process known as venipuncture. This involves inserting a needle into a vein, typically in the arm, to draw a sufficient volume of blood for testing. This step is crucial as the sample will be analyzed for the presence of neutralizing antibodies.
  • Step 3: Laboratory Analysis The collected blood sample is then sent to a laboratory where it undergoes testing using culture and neutralization techniques. This analysis determines the ability of the antibodies present in the sample to neutralize the specific virus being tested.
  • Step 4: Interpretation of Results After the laboratory analysis is complete, the results are interpreted by qualified personnel. A positive result indicates the presence of neutralizing antibodies, suggesting immunity, while a negative result may indicate a lack of sufficient immune response.

3. Post-Procedure

After the neutralization test is completed, patients may be advised on the next steps based on their results. If the test indicates the presence of neutralizing antibodies, the patient may be informed of their immunity status. In cases where the test results are negative, further evaluation or additional vaccinations may be recommended, especially for those at high risk for exposure to the virus. Patients are typically monitored for any adverse reactions following the venipuncture, although complications are rare. It is also important for individuals who are at continuous risk for rabies exposure to adhere to the recommended testing schedule, which may involve retesting every six months or every two years, depending on their level of risk.

Short Descr NEUTRALIZATION TEST VIRAL
Medium Descr NEUTRALIZATION TEST VIRAL
Long Descr Neutralization test, viral
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 3
CCS Clinical Classification 235 - Other Laboratory
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
Date
Action
Notes
2011-01-01 Changed Short description changed.
Pre-1990 Added Code added.
Code
Description
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