CPT® Code 86832

Antibody to human leukocyte antigens (HLA), solid phase assays (eg, microspheres or beads, ELISA, Flow cytometry); high definition qualitative panel for identification of antibody specificities (eg, individual antigen per bead methodology), HLA Class I

The human leukocyte antigen (HLA) system is a critical component of the major histocompatibility complex (MHC), which plays a vital role in the immune system by facilitating self-recognition. This means that the MHC helps the body identify its own cells and tissues while also defending against foreign substances, such as microorganisms and non-self cells. The HLA system is divided into two main classes: Class I and Class II. The testing for HLA antibodies is essential in various clinical scenarios, particularly in the context of organ transplantation. The presence and characteristics of HLA alloantibodies can significantly influence the risk of an immunological response to transplanted organs. CPT® Code 86832 specifically refers to the use of solid phase assays, such as microspheres or beads, enzyme-linked immunosorbent assay (ELISA), and flow cytometry, to conduct a high definition qualitative panel aimed at identifying antibody specificities related to HLA Class I antigens. This test utilizes advanced methodologies, including the individual antigen per bead approach, allowing for the identification of antibodies against a comprehensive panel of cloned Class I HLA antigens. The assay can detect specificities for as many as 80 HLA A/B/C antigens by employing up to 98 beads in the testing array. This detailed identification process is crucial for predicting potential immunological responses in patients undergoing organ transplantation.