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Official Description

Frozen blood, each unit; thawing

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 86931 refers to the laboratory procedure involved in thawing frozen blood, specifically for each unit. This process is essential in the management of blood transfusions, allowing healthcare providers to utilize stored blood units as needed. The procedure begins with the collection of blood from a donor, which is performed as a separately reportable procedure. Once collected, the blood undergoes preparation where serum or plasma is separated from red blood cells. To preserve the integrity of the red blood cells during freezing, they are treated with a glycerol-based cryoprotectant. This preservative penetrates the cell membrane, displacing water within the cells and preventing the formation of ice crystals during the freezing process. Ice crystals can cause damage to the red blood cells, leading to swelling and rupture. The blood unit is then frozen at extremely low temperatures, specifically at -122.8 °F or lower, ensuring that the cells remain viable for future transfusion. When the blood is required for transfusion, it is thawed by placing the unit in a water bath maintained at 98.6°F (37°C) for a duration of 30 minutes. Following thawing, the red blood cells undergo a washing process, known as deglycerolization, using a series of progressively dilute sodium solutions to remove the glycerol, white blood cell fragments, and plasma. After this process, the thawed unit can be stored at temperatures between 1-6°C for up to 24 hours. If the thawed blood is not immediately needed for transfusion, it may be refrozen, provided that a rejuvenation chemical is added prior to refreezing. This code specifically captures the thawing aspect of the blood management process, distinguishing it from other related procedures such as the preparation and freezing of blood units, which are reported under different codes.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure associated with CPT® Code 86931 is indicated for the management of blood transfusions where previously frozen blood units are required. The specific indications for performing this procedure include:

  • Blood Transfusion Needs The thawing of frozen blood is necessary when a patient requires a transfusion of red blood cells that have been stored for future use.
  • Emergency Situations In cases of trauma or significant blood loss, thawed blood units can be quickly made available to meet urgent transfusion requirements.
  • Planned Surgical Procedures Patients scheduled for surgeries that may involve significant blood loss may have frozen blood units thawed in anticipation of transfusion needs during or after the procedure.

2. Procedure

The procedure for thawing frozen blood units as described by CPT® Code 86931 involves several critical steps to ensure the safety and efficacy of the blood for transfusion. The process begins with the retrieval of the frozen blood unit from storage, where it has been maintained at temperatures of -122.8 °F or lower. The frozen unit is then placed in a water bath that is set to a temperature of 98.6°F (37°C). This controlled environment allows for the gradual thawing of the blood, which typically takes about 30 minutes. During this time, it is crucial to monitor the temperature to ensure that the blood is thawed uniformly without causing damage to the red blood cells. Once thawed, the next step involves washing the red blood cells through a process known as deglycerolization. This is achieved by using a series of progressively dilute sodium solutions, specifically 12%, 1.6%, and 0.9% sodium solutions mixed with 2% dextrose. This washing process effectively removes the glycerol preservative that was used during the freezing process, as well as any white blood cell fragments and plasma that may be present. After the deglycerolization is complete, the thawed blood unit can be stored at a temperature range of 1-6°C for a maximum of 24 hours. If the thawed blood is not utilized within this timeframe, it may be refrozen, provided that a rejuvenation chemical is added to the unit prior to refreezing, ensuring the viability of the red blood cells for future transfusion.

3. Post-Procedure

After the thawing and deglycerolization of the blood unit, careful monitoring and handling are essential. The thawed blood must be stored at temperatures between 1-6°C and should be used within 24 hours to ensure its safety and effectiveness for transfusion. If the blood is not needed immediately, it is critical to add a rejuvenation chemical before refreezing to maintain the integrity of the red blood cells. Additionally, healthcare providers must document the thawing process, including the time of thawing and any relevant observations, to ensure compliance with regulatory standards and to facilitate accurate billing and coding. Proper labeling of the thawed blood unit is also necessary to prevent any mix-ups and to ensure that the unit is used appropriately for the intended patient. Overall, adherence to these post-procedure guidelines is vital for maintaining the quality of the blood product and ensuring patient safety during transfusion procedures.

Short Descr FROZEN BLOOD THAW
Medium Descr FROZEN BLOOD EACH UNIT THAWING
Long Descr Frozen blood, each unit; thawing
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
APC Status Indicator STV-Packaged Codes
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 235 - Other Laboratory
Date
Action
Notes
2003-01-01 Changed Code description changed.
1993-01-01 Added First appearance in code book in 1993.
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