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Official Description

Hemolysins and agglutinins; incubated

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 86941 refers to a laboratory test that screens a unit of blood for the presence of hemolysins and cold agglutinins, specifically when the sample is incubated during the testing process. Hemolysins are antibodies that can cause the destruction of red blood cells, leading to hemolytic reactions, which can be dangerous during blood transfusions. Cold agglutinins, on the other hand, are antibodies that can agglutinate red blood cells at lower temperatures, potentially complicating the identification of blood group antigens. This screening is crucial prior to transfusion to ensure the safety and compatibility of the blood being administered to patients. The incubation of the sample during the test is a key aspect of this procedure, as it allows for the optimal detection of these potentially harmful substances, thereby reducing the risk of transfusion-related complications.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure associated with CPT® Code 86941 is indicated for the following conditions:

  • Screening for Hemolysins This test is performed to detect the presence of hemolysins, which are antibodies that can lead to the destruction of red blood cells, posing a risk during blood transfusions.
  • Detection of Cold Agglutinins The procedure is also indicated for identifying cold agglutinins, which can interfere with the accurate identification of blood group antigens, potentially complicating transfusion compatibility.

2. Procedure

The procedure for CPT® Code 86941 involves several critical steps to ensure accurate screening of the blood sample. First, a unit of blood is collected and prepared for testing. The sample is then subjected to an incubation process, which is essential for the detection of hemolysins and cold agglutinins. During this incubation, the blood sample is maintained at a controlled temperature to facilitate the interaction between the antibodies and the red blood cells. This step is crucial as it allows for the optimal conditions under which hemolysins and cold agglutinins can be identified. After the incubation period, the sample is evaluated for the presence of these substances, which can indicate potential risks associated with transfusion. The results of this screening are then documented, providing vital information for healthcare providers to make informed decisions regarding blood transfusion safety.

  • Step 1: Sample Collection A unit of blood is collected and prepared for testing to ensure that it is suitable for screening.
  • Step 2: Incubation The blood sample is incubated under controlled conditions to allow for the detection of hemolysins and cold agglutinins.
  • Step 3: Evaluation After incubation, the sample is evaluated for the presence of hemolysins and cold agglutinins, which are critical for assessing transfusion compatibility.
  • Step 4: Documentation The results of the screening are documented, providing essential information for healthcare providers regarding the safety of the blood for transfusion.

3. Post-Procedure

Post-procedure care following the screening associated with CPT® Code 86941 typically involves monitoring the results to determine the safety of the blood for transfusion. If hemolysins or cold agglutinins are detected, healthcare providers may need to take additional steps to ensure patient safety, which could include selecting alternative blood products or further testing. It is essential for healthcare professionals to communicate the results of the screening to the relevant medical team to facilitate informed decision-making regarding transfusion practices. Additionally, proper documentation of the test results is crucial for compliance and future reference.

Short Descr HEMOLYSINS/AGGLUTININS
Medium Descr HEMOLYSINS&AGGLUTININS INCUBATED
Long Descr Hemolysins and agglutinins; incubated
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 235 - Other Laboratory
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
Date
Action
Notes
1993-01-01 Added First appearance in code book in 1993.
Code
Description
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