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Official Description

Infectious agent antigen detection by immunofluorescent technique; cryptosporidium

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 87272 refers to a laboratory test specifically designed for the detection of cryptosporidium antigen using an immunofluorescent technique. This test is crucial for identifying the presence of the protozoan parasite Cryptosporidium, which is commonly found in contaminated food and water sources. Upon ingestion, Cryptosporidium can invade the intestinal wall, leading to a range of gastrointestinal symptoms such as diarrhea, abdominal pain, fever, nausea, and vomiting. These symptoms typically manifest within a few days and can last from one to two weeks. In healthy individuals with a competent immune system, the infection is often self-limiting, meaning it resolves without the need for extensive medical intervention. However, for those who are immunocompromised, the infection can become persistent and may lead to more severe health complications. The testing process involves obtaining a fecal sample, which is then processed to concentrate the oocytes of the parasite. The sample is fixed onto a slide and treated with antibodies that are conjugated to fluorescein isothiocyanate, allowing for visualization under an epifluorescence microscope. If Cryptosporidium oocytes are present, they will bind to the antibodies, resulting in a bright green fluorescence that indicates a positive test result. This method is recognized for its higher sensitivity compared to other testing methods, such as enzyme immunoassays or direct fecal examinations, making it a preferred choice for accurate diagnosis.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The CPT® Code 87272 is indicated for the detection of cryptosporidium antigen in patients who present with gastrointestinal symptoms that may suggest a cryptosporidial infection. The following conditions warrant the use of this test:

  • Diarrhea Persistent or severe diarrhea that may be associated with exposure to contaminated water or food sources.
  • Abdominal Pain Abdominal discomfort that accompanies gastrointestinal symptoms, particularly in individuals with potential exposure to the parasite.
  • Fever The presence of fever in conjunction with gastrointestinal symptoms, indicating a possible infectious process.
  • Nausea and Vomiting Symptoms of nausea and vomiting that may be indicative of a gastrointestinal infection.
  • Immunocompromised Status Patients with weakened immune systems who are at higher risk for severe or prolonged infections.

2. Procedure

The procedure for performing the test under CPT® Code 87272 involves several critical steps to ensure accurate detection of cryptosporidium antigens:

  • Sample Collection A fecal sample is collected from the patient, which serves as the primary specimen for testing.
  • Centrifugation The fecal sample is then subjected to centrifugation. This process concentrates the oocytes of Cryptosporidium, making them easier to detect in subsequent steps.
  • Slide Preparation After centrifugation, the concentrated sample is fixed onto a microscope slide. This step is essential for preserving the integrity of the oocytes for examination.
  • Inoculation with Antibodies The slide is inoculated with antibodies that are specifically conjugated to fluorescein isothiocyanate. These antibodies are designed to bind to the cryptosporidium antigens present in the sample.
  • Microscopic Examination The prepared slide is examined using an epifluorescence microscope. If cryptosporidium oocytes are present, the antibodies will attach to the antigens, causing the oocytes to fluoresce bright green.
  • Result Interpretation The test is reported as positive if one or more oocytes are detected in the fecal sample, indicating the presence of Cryptosporidium infection.

3. Post-Procedure

After the completion of the test, the results are typically available within a short timeframe, allowing for timely diagnosis and management of the patient's condition. If the test result is positive, further clinical evaluation and treatment may be necessary, especially for immunocompromised patients who may require more intensive medical intervention. It is important for healthcare providers to monitor the patient's symptoms and overall health following the test, as cryptosporidial infections can lead to complications if not addressed appropriately. Additionally, proper hygiene and sanitation measures should be reinforced to prevent the spread of the infection, particularly in settings where individuals may be at higher risk.

Short Descr CRYPTOSPORIDIUM AG IF
Medium Descr IAADI CRYPTOSPORIDIUM
Long Descr Infectious agent antigen detection by immunofluorescent technique; cryptosporidium
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
GA Waiver of liability statement issued as required by payer policy, individual case
Date
Action
Notes
2011-01-01 Changed Short description changed.
2004-01-01 Changed Code description changed.
2001-01-01 Changed Code description changed.
1998-01-01 Added First appearance in code book in 1998.
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