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Official Description

Infectious agent antigen detection by immunofluorescent technique, polyvalent for multiple organisms, each polyvalent antiserum

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 87300 refers to the detection of infectious agents through an immunofluorescent technique, utilizing a polyvalent approach for multiple organisms. This procedure involves the collection of a tissue sample from the patient, which is then treated with a fluoroscopic dye. The purpose of this dye is to enhance the visibility of the sample when examined under a fluorescent microscope. By using this method, healthcare professionals can identify the presence of various pathogenic organisms within the sample. The polyvalent antiserum used in this technique is designed to react with multiple types of infectious agents, allowing for a broader assessment of potential infections. This procedure is particularly valuable in diagnosing infections caused by a range of pathogens, as it provides a rapid and effective means of detection, facilitating timely medical intervention.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure associated with CPT® Code 87300 is indicated for the detection of infectious agents in various clinical scenarios. The following conditions may warrant the use of this immunofluorescent technique:

  • Infectious Disease Diagnosis The procedure is performed to identify the presence of multiple pathogenic organisms in a tissue sample, aiding in the diagnosis of infectious diseases.
  • Unexplained Symptoms It may be indicated in patients presenting with unexplained symptoms that suggest an underlying infectious process, where traditional diagnostic methods may not provide conclusive results.
  • Monitoring Treatment Efficacy This technique can also be utilized to monitor the effectiveness of ongoing treatment for infections, allowing for adjustments in therapeutic strategies based on the detection of specific pathogens.

2. Procedure

The procedure for CPT® Code 87300 involves several key steps to ensure accurate detection of infectious agents. Each step is critical to the overall effectiveness of the diagnostic process.

  • Step 1: Sample Collection A tissue sample is collected from the patient, which may involve a biopsy or aspiration, depending on the clinical context. The sample must be handled carefully to preserve its integrity for subsequent analysis.
  • Step 2: Application of Fluoroscopic Dye Once the tissue sample is obtained, a fluoroscopic dye is applied. This dye is essential for enhancing the visibility of the sample under a fluorescent microscope, as it binds to the antigens present in the infectious agents.
  • Step 3: Microscopic Examination The treated sample is then examined under a fluorescent microscope. The healthcare professional looks for specific fluorescence patterns that indicate the presence of multiple pathogenic organisms, as the dye will emit light when bound to the antigens of these organisms.

3. Post-Procedure

After the procedure associated with CPT® Code 87300, the patient may require monitoring for any immediate reactions to the tissue collection. The results of the immunofluorescent examination will typically be available within a specified timeframe, allowing for timely diagnosis and treatment decisions. It is important for healthcare providers to communicate the findings to the patient and discuss any necessary follow-up actions or additional testing that may be required based on the results. Additionally, proper documentation of the procedure and findings is essential for compliance and billing purposes.

Short Descr AG DETECTION POLYVAL IF
Medium Descr IAADI POLYV MLT ORGANISMS EA POLYV ANTISERUM
Long Descr Infectious agent antigen detection by immunofluorescent technique, polyvalent for multiple organisms, each polyvalent antiserum
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 2
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
Date
Action
Notes
2011-01-01 Changed Short description changed.
2001-01-01 Added First appearance in code book in 2001.
1983-12-31 Deleted Code deleted.
Code
Description
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