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Official Description

Infectious agent detection by nucleic acid (DNA or RNA); central nervous system pathogen (eg, Neisseria meningitidis, Streptococcus pneumoniae, Listeria, Haemophilus influenzae, E. coli, Streptococcus agalactiae, enterovirus, human parechovirus, herpes simplex virus type 1 and 2, human herpesvirus 6, cytomegalovirus, varicella zoster virus, Cryptococcus), includes multiplex reverse transcription, when performed, and multiplex amplified probe technique, multiple types or subtypes, 12-25 targets

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 87483 refers to a laboratory test designed for the detection of infectious agents within the central nervous system (CNS) by analyzing nucleic acids, specifically DNA or RNA. This test is crucial for identifying various pathogens that can cause serious CNS infections, such as Neisseria meningitidis, Streptococcus pneumoniae, Listeria, Haemophilus influenzae, E. coli, Streptococcus agalactiae, enterovirus, human parechovirus, herpes simplex virus types 1 and 2, human herpesvirus 6, cytomegalovirus, varicella zoster virus, and Cryptococcus. The complexity of diagnosing CNS infections arises from the overlapping symptoms with other conditions, including meningitis, encephalitis, and brain mass lesions, which can be caused by bacteria, viruses, fungi, or parasites. Traditional methods of pathogen detection, such as culture or antigen-antibody based techniques, may be slower and less sensitive compared to this targeted nucleic acid testing. The procedure involves collecting cerebrospinal fluid (CSF) through a lumbar puncture, which is a separate reportable procedure. The analysis of the CSF sample is performed using a multiplex reverse transcription and multiplex amplified probe technique, allowing for the simultaneous detection of multiple types or subtypes of pathogens, typically ranging from 12 to 25 targets. This advanced testing method enhances the speed and accuracy of diagnosing CNS infections, facilitating timely and appropriate treatment for affected patients.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The CPT® Code 87483 is indicated for use in the detection of various central nervous system pathogens. The specific indications for this procedure include:

  • Neisseria meningitidis - A bacterium that can cause meningitis and other serious infections.
  • Streptococcus pneumoniae - A common cause of bacterial meningitis and pneumonia.
  • Listeria - A bacterium that can lead to meningitis, particularly in pregnant women and immunocompromised individuals.
  • Haemophilus influenzae - A bacterium that can cause severe infections, including meningitis, especially in children.
  • E. coli - Certain strains can lead to CNS infections, particularly in neonates.
  • Streptococcus agalactiae - Also known as Group B Streptococcus, it can cause meningitis in newborns.
  • Enterovirus - A group of viruses that can cause viral meningitis.
  • Human parechovirus - A virus that can lead to CNS infections, particularly in infants.
  • Herpes simplex virus type 1 and 2 - Viruses that can cause encephalitis and meningitis.
  • Human herpesvirus 6 - Associated with CNS infections, particularly in children.
  • Cytomegalovirus - Can cause CNS infections, especially in immunocompromised patients.
  • Varicella zoster virus - The virus responsible for chickenpox and shingles, which can also affect the CNS.
  • Cryptococcus - A fungus that can cause meningitis, particularly in immunocompromised individuals.

2. Procedure

The procedure for CPT® Code 87483 involves several critical steps to ensure accurate detection of CNS pathogens. The process begins with the collection of cerebrospinal fluid (CSF) through a lumbar puncture, which is a separate procedure that must be reported independently. Once the CSF sample is obtained, it undergoes a series of processing steps. In the first step, the DNA present in the sample is denatured using heat, which separates the double-stranded DNA into single strands. This is a crucial step as it prepares the DNA for amplification. In the second step, polymerase chain reaction (PCR) primers, which are short DNA segments consisting of 20-30 base paired oligonucleotides, are introduced. These primers are complementary to the ends of the DNA double strands and facilitate the amplification of specific DNA sequences. The third step involves synthesizing complementary strands of DNA in the presence of enzymes, which is repeated multiple times to produce millions of copies of the target DNA. This amplification process is essential for detecting even small amounts of pathogen DNA or RNA in the CSF sample. Finally, the amplified DNA is analyzed using specialized machines that identify the presence of specific nucleic acids, confirming the presence of the pathogens tested for.

3. Post-Procedure

After the completion of the procedure associated with CPT® Code 87483, the CSF sample is analyzed, and results are typically available within a short timeframe, allowing for rapid diagnosis of CNS infections. Post-procedure care primarily involves monitoring the patient for any potential complications related to the lumbar puncture, such as headache or infection at the puncture site. Patients may be advised to rest and stay hydrated following the procedure. The results of the nucleic acid detection test will guide clinicians in determining the appropriate treatment plan based on the identified pathogens. It is essential for healthcare providers to communicate the results to the patient and discuss any necessary follow-up care or additional testing that may be required.

Short Descr CNS DNA AMP PROBE TYPE 12-25
Medium Descr CNS DNA/RNA AMP PROBE MULTIPLE SUBTYPES 12-25
Long Descr Infectious agent detection by nucleic acid (DNA or RNA); central nervous system pathogen (eg, Neisseria meningitidis, Streptococcus pneumoniae, Listeria, Haemophilus influenzae, E. coli, Streptococcus agalactiae, enterovirus, human parechovirus, herpes simplex virus type 1 and 2, human herpesvirus 6, cytomegalovirus, varicella zoster virus, Cryptococcus), includes multiplex reverse transcription, when performed, and multiplex amplified probe technique, multiple types or subtypes, 12-25 targets
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T2D - Other tests - other
MUE 1
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
T5 Right foot, great toe
GW Service not related to the hospice patient's terminal condition
TA Left foot, great toe
T1 Left foot, second digit
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
GZ Item or service expected to be denied as not reasonable and necessary
T2 Left foot, third digit
T3 Left foot, fourth digit
T4 Left foot, fifth digit
T6 Right foot, second digit
T7 Right foot, third digit
T8 Right foot, fourth digit
T9 Right foot, fifth digit
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2017-01-01 Added Added
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