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Official Description

Infectious agent detection by nucleic acid (DNA or RNA); hepatitis G, direct probe technique

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 87525 refers to a laboratory test designed for the detection of the hepatitis G virus (HGV), also known as GB virus-C (GBV-C), through the use of nucleic acid techniques, specifically targeting DNA or RNA. This test employs a direct probe technique to identify the unique nucleic acid sequence of the hepatitis G virus present in a blood sample. HGV infections are often asymptomatic and do not typically lead to significant liver inflammation; however, they can occur alongside other viral infections that do cause acute or chronic hepatitis. The transmission of HGV primarily occurs through infected blood and blood products, from mother to infant during childbirth, and through sexual contact with an infected partner. The direct probe test utilizes a labeled probe that binds specifically to the target sequence of the hepatitis G virus, allowing for its detection if present in the sample. The probe is equipped with fluorescent or chemiluminescent labels, enhancing the visibility of the target sequence. The process involves treating the sample to release nucleic acids from the virus, followed by the formation of a stable hybrid between the probe and the target sequence. Ribosomal RNA is often the focus of these tests due to its abundance in microorganisms, which allows for more effective detection compared to genomic DNA. For enhanced sensitivity, an amplified probe technique (CPT® Code 87526) may be employed, which involves exponentially increasing the target sequence of the hepatitis G virus DNA or RNA prior to detection. This amplification is commonly achieved through polymerase chain reaction (PCR) or reverse transcriptase polymerase chain reaction (RT-PCR). Furthermore, nucleic acid detection with quantification (CPT® Code 87527) provides an assessment of the quantity of microorganisms present, utilizing quantitative or real-time PCR to generate detailed reports on the amounts of the detected nucleic acid sequences.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The CPT® Code 87525 is indicated for the detection of the hepatitis G virus (HGV) in patients who may be at risk for infection. The following conditions and situations warrant the use of this test:

  • Asymptomatic Infection Patients who may be carriers of the hepatitis G virus without exhibiting symptoms.
  • Co-infection with Other Hepatitis Viruses Individuals who are co-infected with other hepatitis viruses, such as hepatitis B or C, where HGV may complicate the clinical picture.
  • Blood Transfusion Recipients Individuals who have received blood transfusions or blood products, as HGV can be transmitted through infected blood.
  • Pregnant Women Pregnant women who may transmit the virus to their infants during childbirth.
  • High-Risk Sexual Partners Individuals with sexual partners who are known to be infected with HGV.

2. Procedure

The procedure for CPT® Code 87525 involves several critical steps to ensure accurate detection of the hepatitis G virus. The following outlines the procedural steps:

  • Sample Collection A blood sample is collected from the patient, which serves as the specimen for testing. Proper collection techniques must be followed to avoid contamination and ensure the integrity of the sample.
  • Nucleic Acid Extraction The collected blood sample is treated to release nucleic acids from any present hepatitis G virus. This step is crucial as it prepares the sample for the subsequent detection process.
  • Probe Hybridization A labeled probe, which is specific to the target sequence of the hepatitis G virus, is introduced to the sample. The probe binds to the unique nucleic acid sequence of the virus, forming a stable hybrid if the target is present.
  • Detection of Hybridization The hybridized probe is then detected using fluorescent or chemiluminescent methods. This detection allows for the identification of the hepatitis G virus in the sample.

3. Post-Procedure

After the completion of the procedure associated with CPT® Code 87525, the laboratory will analyze the results and generate a report detailing the presence or absence of the hepatitis G virus in the sample. If the virus is detected, further clinical evaluation and management may be necessary, depending on the patient's overall health and any co-existing conditions. It is important for healthcare providers to discuss the results with the patient and consider any implications for treatment or further testing. Additionally, proper documentation of the procedure and results is essential for compliance and billing purposes.

Short Descr HEPATITIS G DNA DIR PROBE
Medium Descr IADNA HEPATITIS G DIRECT PROBE TECHNIQUE
Long Descr Infectious agent detection by nucleic acid (DNA or RNA); hepatitis G, direct probe technique
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
Date
Action
Notes
2011-01-01 Changed Short description changed.
1998-01-01 Added First appearance in code book in 1998.
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Description
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Description
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