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Official Description

Infectious agent detection by nucleic acid (DNA or RNA); Herpes simplex virus, amplified probe technique

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 87529 refers to the detection of infectious agents, specifically the Herpes simplex virus (HSV), through nucleic acid techniques that amplify the genetic material (DNA or RNA) of the virus. This method utilizes an amplified probe technique, which is particularly useful in cases where the viral load may be low, making it difficult to detect the virus using standard direct probe methods. Herpes simplex virus is known to be transmitted through direct contact, and the initial infection often manifests as ulcerations on the skin or mucous membranes. After the primary infection, the virus can enter a dormant state within the body and may reactivate due to various triggers, including other illnesses, infections, or stress. Individuals at higher risk for severe complications from HSV include neonates and those with compromised immune systems, as they may develop serious infections affecting the eyes or central nervous system. There are two primary types of HSV: type 1, which is commonly associated with oral herpes (cold sores) but can also cause genital infections, and type 2, which primarily leads to genital herpes. The testing process involves obtaining a specimen from lesions, vitreous fluid from the eye, or blood samples, and employing amplification techniques such as polymerase chain reaction (PCR) to enhance the detection of HSV nucleic acids. This amplified detection is crucial for accurately identifying the presence of the virus, especially in cases where it may be present in low quantities.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure associated with CPT® Code 87529 is indicated for the detection of Herpes simplex virus (HSV) in various clinical scenarios. The following conditions warrant the use of this testing method:

  • Primary Infection: When a patient presents with symptoms indicative of a primary HSV infection, such as ulcerations in the skin or mucous membranes.
  • Reactivation of Virus: In cases where there is a suspicion of reactivation of HSV due to other illnesses, infections, or stress factors.
  • High-Risk Populations: Testing is particularly important for neonates and immunocompromised individuals who are at increased risk for severe complications, including ocular or central nervous system infections.
  • Diagnostic Confirmation: To confirm the presence of HSV in patients with clinical signs suggestive of herpes infection.

2. Procedure

The procedure for CPT® Code 87529 involves several critical steps to ensure accurate detection of the Herpes simplex virus through amplification of its nucleic acids. The following procedural steps are outlined:

  • Specimen Collection: A specimen is obtained from a relevant source, which may include an oral or genital lesion, vitreous fluid from the eye, or a blood sample. This specimen serves as the basis for testing.
  • Amplification Technique: The collected specimen undergoes an amplification process, such as polymerase chain reaction (PCR). This technique is employed to create multiple copies of the HSV-1 and/or HSV-2 nucleic acids present in the specimen, which is particularly useful when the viral load is suspected to be low.
  • Detection of Nucleic Acids: Following amplification, various detection techniques are utilized to identify the presence of the amplified HSV nucleic acids. This step is crucial for confirming the presence of the virus in the specimen.

3. Post-Procedure

After the procedure associated with CPT® Code 87529, the results of the test will be analyzed to determine the presence of Herpes simplex virus. Depending on the findings, further clinical management may be required. If quantification of the viral load is performed, it can provide valuable information regarding the severity of the infection and the patient's response to treatment. It is essential for healthcare providers to interpret the results in conjunction with clinical findings and patient history to ensure appropriate follow-up and management of the infection.

Short Descr HSV DNA AMP PROBE
Medium Descr IADNA HERPES SOMPLX VIRUS AMPLIFIED PROBE TQ
Long Descr Infectious agent detection by nucleic acid (DNA or RNA); Herpes simplex virus, amplified probe technique
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 2
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
GW Service not related to the hospice patient's terminal condition
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
GV Attending physician not employed or paid under arrangement by the patient's hospice provider
Q4 Service for ordering/referring physician qualifies as a service exemption
Q6 Service furnished under a fee-for-time compensation arrangement by a substitute physician or by a substitute physical therapist furnishing outpatient physical therapy services in a health professional shortage area, a medically underserved area, or a rural area
X2 Continuous/focused services: for reporting services by clinicians whose expertise is needed for the ongoing management of a chronic disease or a condition that needs to be managed and followed with no planned endpoint to the relationship; reporting clinician service examples include but are not limited to: a rheumatologist taking care of the patient's rheumatoid arthritis longitudinally but not providing general primary care services
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
76 Repeat procedure or service by same physician or other qualified health care professional: it may be necessary to indicate that a procedure or service was repeated by the same physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 76 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
77 Repeat procedure by another physician or other qualified health care professional: it may be necessary to indicate that a basic procedure or service was repeated by another physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 77 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
95 Synchronous telemedicine service rendered via a real-time interactive audio and video telecommunications system: synchronous telemedicine service is defined as a real-time interaction between a physician or other qualified health care professional and a patient who is located at a distant site from the physician or other qualified health care professional. the totality of the communication of information exchanged between the physician or other qualified health care professional and the patient during the course of the synchronous telemedicine service must be of an amount and nature that would be sufficient to meet the key components and/or requirements of the same service when rendered via a face-to-face interaction. modifier 95 may only be appended to the services listed in appendix p. appendix p is the list of cpt codes for services that are typically performed face-to-face, but may be rendered via a real-time (synchronous) interactive audio and video telecommunications system.
GA Waiver of liability statement issued as required by payer policy, individual case
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
GZ Item or service expected to be denied as not reasonable and necessary
KX Requirements specified in the medical policy have been met
RT Right side (used to identify procedures performed on the right side of the body)
SA Nurse practitioner rendering service in collaboration with a physician
U2 Medicaid level of care 2, as defined by each state
XE Separate encounter, a service that is distinct because it occurred during a separate encounter
XS Separate structure, a service that is distinct because it was performed on a separate organ/structure
Date
Action
Notes
2011-01-01 Changed Short description changed.
1998-01-01 Added First appearance in code book in 1998.
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