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Official Description

Infectious agent detection by nucleic acid (DNA or RNA); HIV-2, direct probe technique

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 87537 refers to the detection of the infectious agent HIV-2 through nucleic acid techniques, specifically utilizing a direct probe method. This procedure is essential for identifying the presence of HIV-2, one of the two serotypes of the human immunodeficiency virus, which can lead to acquired immunodeficiency syndrome (AIDS). HIV-2 is primarily transmitted through sexual contact, but it can also be spread through unscreened blood transfusions, sharing of needles, from mother to child during pregnancy or childbirth, and through breastfeeding. The virus targets the immune system by attacking T-cells, also known as CD4 cells, which are crucial for the body's defense against infections. The testing process begins with obtaining a blood sample from the patient. In the context of CPT® Code 87537, a direct probe technique is employed, which is less common due to its requirement for culturing the specimen. This method contrasts with other techniques such as amplified probe methods, which utilize amplification processes like reverse transcription polymerase chain reaction (RT-PCR) to detect lower levels of the virus. The direct probe technique involves several steps, including the lysis of red blood cells to isolate leukocytes, washing the leukocytes, and extracting DNA from the resulting pellet. The presence of HIV-2 DNA is then determined using specific detection methods. This testing is critical for diagnosing HIV-2 infections and monitoring the viral load, which is essential for evaluating treatment responses.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure associated with CPT® Code 87537 is indicated for the detection of HIV-2 in patients who may be at risk for this specific serotype of the human immunodeficiency virus. The following conditions or situations warrant the use of this testing:

  • High-Risk Sexual Behavior Individuals who engage in unprotected sexual activities with multiple partners or with partners known to be HIV-positive.
  • History of Blood Transfusions Patients who have received unscreened blood products, particularly in regions where HIV-2 is prevalent.
  • Intravenous Drug Use Individuals who share needles or other drug paraphernalia, increasing the risk of HIV transmission.
  • Mother-to-Child Transmission Pregnant women who are HIV-positive or at risk for HIV-2 should be tested to prevent transmission to the fetus during pregnancy or childbirth.
  • Symptoms of HIV Infection Patients presenting with symptoms consistent with HIV infection, such as unexplained weight loss, persistent fever, or recurrent infections.

2. Procedure

The procedure for CPT® Code 87537 involves several critical steps to accurately detect HIV-2 using a direct probe technique. The following outlines the procedural steps:

  • Step 1: Sample Collection A blood sample is obtained from the patient, which serves as the specimen for testing. Proper collection techniques are essential to ensure the integrity of the sample.
  • Step 2: Specimen Preparation The collected blood sample undergoes processing where red blood cells are selectively lysed. This process allows for the isolation of leukocytes, which are the target cells for HIV-2 detection.
  • Step 3: Washing and Pelleting The leukocytes are then pelleted and washed multiple times to remove any contaminants and prepare them for DNA extraction.
  • Step 4: DNA Extraction DNA is extracted from the pelleted leukocytes. This step is crucial as it isolates the proviral HIV-2 DNA that may be present in the sample.
  • Step 5: Direct Probe Technique The extracted DNA is subjected to the direct probe technique, which involves using specific probes to detect the presence of HIV-2 DNA. This method requires culturing the specimen, which is less common compared to amplification techniques.
  • Step 6: Detection The final step involves using detection methods to determine whether HIV-2 DNA is present in the specimen. This confirms the diagnosis of HIV-2 infection based on the results obtained from the direct probe analysis.

3. Post-Procedure

After the completion of the procedure associated with CPT® Code 87537, the patient may be advised on several post-procedure considerations. It is important to monitor for any potential side effects or complications related to the blood draw, such as bruising or discomfort at the puncture site. Additionally, patients should be informed about the timeline for receiving test results, which can vary based on laboratory processing times. If the test result is positive for HIV-2, further counseling and follow-up testing may be necessary to confirm the diagnosis and assess the viral load. Patients should also be educated on the importance of safe practices to prevent the transmission of HIV and the need for regular medical follow-up to manage their health effectively.

Short Descr HIV-2 DNA DIR PROBE
Medium Descr IADNA HIV-2 DIRECT PROBE TECHNIQUE
Long Descr Infectious agent detection by nucleic acid (DNA or RNA); HIV-2, direct probe technique
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
Date
Action
Notes
2011-01-01 Changed Short description changed.
1998-01-01 Added First appearance in code book in 1998.
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