CPT® Code 87660

Infectious agent detection by nucleic acid (DNA or RNA) for Trichomonas vaginalis is a diagnostic procedure aimed at identifying the presence of this specific protozoan parasite, which is responsible for the sexually transmitted infection known as trichomoniasis. Trichomonas vaginalis (T. vaginalis) is a single-celled organism that can lead to various symptoms, including vaginal itching, burning sensations, abnormal discharge, discomfort during sexual intercourse, and painful urination. The procedure utilizes a direct probe technique, which is a form of nucleic acid testing that allows for the rapid detection of the pathogen. This method can often be performed in a physician's office using a specialized test kit. The process begins with the collection of a vaginal swab, which is then treated with a lysate to break down cellular structures and release nucleic acids. Following this, the lysate is introduced into a sample well where a T. vaginalis-specific probe is attached to a stationary microparticle bead. This bead captures the target DNA sequence, facilitating the identification of the organism. Subsequent washing steps remove any unbound materials, and the addition of a second probe with a color development substrate allows for visual confirmation of the test results. A color change indicates the presence of T. vaginalis, while the absence of color signifies a negative result.