CPT® Code 87661

Infectious agent detection by nucleic acid (DNA or RNA) for Trichomonas vaginalis using the amplified probe technique is a diagnostic procedure aimed at identifying the presence of this specific protozoan organism. Trichomonas vaginalis is a single-cell protozoan that is primarily responsible for causing vaginitis, a condition characterized by inflammation of the vagina. This organism is transmitted through sexual contact and can infect both the urethra and vagina, leading to various symptoms associated with sexually transmitted infections. The amplified probe technique allows for rapid and direct identification of Trichomonas vaginalis by analyzing nucleic acids extracted from patient samples. This method is particularly valuable as it can definitively diagnose a trichomonas vaginalis infection, even when other organisms and host DNA are present in the sample. The procedure involves obtaining a vaginal swab, which is then treated to rupture cells and release nucleic acids. The technique relies on the detection of a specific nucleotide sequence within the clinical sample, utilizing a radiolabeled or nonisotopically labeled DNA probe. This probe is designed to bind with the target DNA, forming stable complexes that can be detected. The amplified probe technique enhances sensitivity and specificity for qualitative detection by employing methods such as polymerase chain reaction (PCR), which enzymatically replicates target DNA sequences to increase their quantity for easier identification. Additionally, amplification primers are used to ensure that the correct DNA fragments are targeted and replicated, further improving the accuracy of the test.