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Official Description

Tissue culture for non-neoplastic disorders; skin or other solid tissue biopsy

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

A tissue culture is a laboratory procedure that involves obtaining a sample of tissue, such as skin or other solid tissues, to facilitate a chromosome analysis specifically for non-neoplastic disorders. Non-neoplastic disorders refer to genetic conditions that do not involve tumors or cancerous growths but may include a variety of genetic anomalies. These can encompass conditions such as sister chromatid exchange (SCE), breakage syndromes, trisomies, and mosaicism, among others. The purpose of this procedure is to promote cell growth from the biopsy sample, allowing for detailed genetic analysis. The process begins with the collection of a tissue biopsy, which is then cultured in a controlled environment to encourage the proliferation of cells. This is similar to the procedure outlined in CPT® Code 88230, where lymphocytes from a peripheral blood sample are cultured. In both cases, the cultured cells are subjected to specific treatments to prepare them for chromosome analysis, ensuring that a sufficient number of mitotic cells are available for accurate evaluation. The results of this analysis can provide critical insights into congenital or other genetic conditions that may affect the patient.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure is indicated for the evaluation of various non-neoplastic disorders, which may include the following conditions:

  • Sister Chromatid Exchange (SCE) - A genetic phenomenon where sister chromatids exchange genetic material, which can be indicative of certain genetic disorders.
  • Breakage Syndromes - A group of genetic disorders characterized by an increased susceptibility to chromosome breakage, leading to various clinical manifestations.
  • Trisomies - Conditions resulting from the presence of an extra chromosome, which can lead to developmental and physical abnormalities.
  • Mosaicism - A condition where an individual has two or more genetically different cell lines, which can result in a variety of clinical presentations.
  • Other Genetic Conditions - This includes a range of congenital anomalies and genetic disorders that may not fall into the above categories but require chromosome analysis for diagnosis.

2. Procedure

The procedure for CPT® Code 88233 involves several key steps to ensure the successful culture of skin or other solid tissue for chromosome analysis:

  • Step 1: Biopsy Collection - A biopsy of the skin or other solid tissue is performed to obtain a sample. This involves using sterile techniques to minimize contamination and ensure the integrity of the tissue sample.
  • Step 2: Tissue Culture Preparation - The collected tissue is then placed in a suitable culture medium that promotes cell growth. This environment is carefully controlled to provide the necessary nutrients and conditions for the cells to proliferate.
  • Step 3: Cell Stimulation - Similar to the lymphocyte culture process described in CPT® Code 88230, the cultured cells may be stimulated to divide using specific agents. This step is crucial for ensuring that a sufficient number of cells are available for analysis.
  • Step 4: Mitotic Blockage - A spindle inhibitor is added to the culture to halt the cells in metaphase, which is essential for chromosome analysis. This ensures that the chromosomes are well-defined and can be accurately evaluated under a microscope.
  • Step 5: Staining and Analysis - Depending on the specific requirements of the chromosome analysis, various stains or chemicals may be applied to the cultured cells. This enhances the visibility of the chromosomes, allowing for detailed examination and identification of any genetic abnormalities.

3. Post-Procedure

After the tissue culture procedure, the cultured cells are monitored for growth and viability. The expected recovery involves allowing sufficient time for the cells to proliferate before proceeding with chromosome analysis. Additional considerations include ensuring that the cultured cells are properly preserved and transported to the laboratory for further testing. The results of the chromosome analysis will provide valuable information regarding the presence of any genetic disorders or anomalies, which can guide further clinical management and decision-making.

Short Descr TISSUE CULTURE SKIN/BIOPSY
Medium Descr TISS CUL NON-NEO DISORDERS SKN/OTH SOLID TISS BX
Long Descr Tissue culture for non-neoplastic disorders; skin or other solid tissue biopsy
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 2
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
GZ Item or service expected to be denied as not reasonable and necessary
Date
Action
Notes
2011-01-01 Changed Short description changed.
Pre-1990 Added Code added.
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