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Official Description

Chromosome analysis; analyze 20-25 cells

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

CPT® Code 88264 refers to the procedure of chromosome analysis, specifically analyzing 20 to 25 cells. This analysis is typically performed using peripheral blood lymphocytes, skin, or solid tissue that has been cultured in a separately reportable procedure. The primary goal of this analysis is to evaluate the chromosomal composition of the sampled cells, which involves assessing the size, shape, and number of chromosomes present. Unlike other related codes, such as 88261, 88262, and 88263, which involve karyotyping and the analysis of a greater number of cells, CPT® 88264 focuses on the analysis of a smaller subset of cells without the detailed karyotyping process. This procedure is essential for identifying any extra, missing, or abnormal chromosome pieces that may be present in the sample. The normal chromosomal configurations for females and males are 46 XX and 46 XY, respectively. Chromosome analysis is a critical diagnostic tool in genetics, as it can reveal chromosomal abnormalities that may lead to various medical conditions, although the specific details of those conditions are not covered under this code.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure associated with CPT® Code 88264 is indicated for the analysis of chromosomal abnormalities in patients who may present with various genetic conditions. The following are specific indications for performing this chromosome analysis:

  • Genetic Disorders Patients suspected of having genetic disorders due to chromosomal abnormalities may require this analysis to confirm or rule out specific conditions.
  • Infertility Individuals experiencing unexplained infertility may undergo chromosome analysis to identify potential chromosomal issues that could affect reproductive health.
  • Recurrent Pregnancy Loss Women with a history of recurrent pregnancy loss may be evaluated for chromosomal abnormalities that could contribute to their condition.
  • Congenital Anomalies Newborns or children presenting with congenital anomalies may be tested to determine if chromosomal abnormalities are a contributing factor.

2. Procedure

The procedure for CPT® Code 88264 involves several key steps to ensure accurate chromosome analysis. The following outlines the procedural steps:

  • Sample Collection A sample is collected from the patient, which may include peripheral blood lymphocytes, skin, or solid tissue. This sample is essential for the subsequent analysis of chromosomes.
  • Cell Culture The collected sample is cultured in a laboratory setting. This step is crucial as it allows for the proliferation of cells, ensuring that there are enough cells available for analysis.
  • Cell Harvesting After an appropriate culture period, typically 24 to 48 hours, the cells are harvested. This involves stopping the cell division process and preparing the cells for analysis.
  • Chromosome Analysis A total of 20 to 25 cells are analyzed for chromosomal composition. This analysis focuses on identifying any extra, missing, or abnormal chromosome pieces without performing karyotyping.
  • Documentation The results of the chromosome analysis are documented, detailing any abnormalities found in the analyzed cells. This documentation is critical for further clinical decision-making.

3. Post-Procedure

After the chromosome analysis is completed, the results are reviewed and interpreted by a qualified healthcare professional. Patients may receive counseling regarding the findings, especially if chromosomal abnormalities are detected. Follow-up care may be necessary depending on the results, which could include additional testing or referrals to genetic specialists. It is important for healthcare providers to discuss the implications of the findings with the patient and consider any further diagnostic or therapeutic options that may be warranted based on the analysis results.

Short Descr CHROMOSOME ANALYSIS 20-25
Medium Descr CHRMSM ANALYZE 20-25 CELLS
Long Descr Chromosome analysis; analyze 20-25 cells
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
GZ Item or service expected to be denied as not reasonable and necessary
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
GA Waiver of liability statement issued as required by payer policy, individual case
52 Reduced services: under certain circumstances a service or procedure is partially reduced or eliminated at the discretion of the physician or other qualified health care professional. under these circumstances the service provided can be identified by its usual procedure number and the addition of modifier 52, signifying that the service is reduced. this provides a means of reporting reduced services without disturbing the identification of the basic service. note: for hospital outpatient reporting of a previously scheduled procedure/service that is partially reduced or cancelled as a result of extenuating circumstances or those that threaten the well-being of the patient prior to or after administration of anesthesia, see modifiers 73 and 74 (see modifiers approved for asc hospital outpatient use).
77 Repeat procedure by another physician or other qualified health care professional: it may be necessary to indicate that a basic procedure or service was repeated by another physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 77 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
AQ Physician providing a service in an unlisted health professional shortage area (hpsa)
GC This service has been performed in part by a resident under the direction of a teaching physician
GW Service not related to the hospice patient's terminal condition
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
Q1 Routine clinical service provided in a clinical research study that is in an approved clinical research study
Q6 Service furnished under a fee-for-time compensation arrangement by a substitute physician or by a substitute physical therapist furnishing outpatient physical therapy services in a health professional shortage area, a medically underserved area, or a rural area
TC Technical component; under certain circumstances, a charge may be made for the technical component alone; under those circumstances the technical component charge is identified by adding modifier 'tc' to the usual procedure number; technical component charges are institutional charges and not billed separately by physicians; however, portable x-ray suppliers only bill for technical component and should utilize modifier tc; the charge data from portable x-ray suppliers will then be used to build customary and prevailing profiles
XS Separate structure, a service that is distinct because it was performed on a separate organ/structure
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
Date
Action
Notes
2011-01-01 Changed Short description changed.
1999-01-01 Added First appearance in code book in 1999.
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