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Official Description

Molecular cytogenetics; chromosomal in situ hybridization, analyze 10-30 cells (eg, for microdeletions)

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 88273 refers to a laboratory test that focuses on molecular cytogenetics, specifically utilizing chromosomal in situ hybridization (CISH) to analyze a sample of 10 to 30 cells. This procedure is essential for identifying chromosomal abnormalities, particularly microdeletions, which are small deletions of chromosomal material that can involve one or more genes and are often linked to various congenital anomalies. The test is typically performed on blood or bone marrow samples, which are obtained through a venipuncture or bone marrow aspiration, both of which are separately reportable procedures. The analysis aims to provide insights into the structure and functional organization of chromosomes, as well as to detect derivatives and markers such as small supernumerary marker chromosomes (sSMCs) and chromosomal exchange material, which can be identified through G-banded chromosome analysis. The results of this test can help in diagnosing several genetic conditions, including but not limited to Angelman syndrome, Prader-Willi syndrome, and Williams syndrome, among others. By analyzing the specified number of cells, this procedure enhances the understanding of chromosome biology and aids in the identification of genetic disorders associated with chromosomal abnormalities.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure associated with CPT® Code 88273 is indicated for the analysis of various genetic conditions that may arise from chromosomal abnormalities. The following conditions may be identified through this molecular cytogenetics test:

  • 15q11.2-13 duplication Identifies duplications in the chromosomal region that may lead to developmental issues.
  • Angelman syndrome A genetic disorder characterized by severe intellectual and developmental disabilities, often accompanied by problems with movement and balance.
  • Crit-du-chat (5p-) syndrome A condition caused by a deletion of a portion of chromosome 5, leading to distinctive facial features and developmental delays.
  • DeGeorge/Velocardiofacial syndrome (22q11.2) A genetic disorder that can cause heart defects, cleft palate, and immune system issues.
  • Kallmann syndrome (Xp22.3) A condition that affects the development of the reproductive system and sense of smell.
  • Miller-Dieker (Lissencephaly) syndrome (17p13.3) A disorder characterized by smooth brain (lissencephaly) and associated developmental delays.
  • Phelan-McDermid (22qter) syndrome (22q13.3) A genetic condition that can lead to developmental delays and autism spectrum disorders.
  • Prader-Willi syndrome (15q11.2-13) A genetic disorder that results in a variety of physical, mental, and behavioral problems, including obesity and developmental delays.
  • SHOX (Xp22.3) A gene associated with skeletal development, and its abnormalities can lead to short stature and other skeletal issues.
  • Smith-Magenis syndrome (17p11.2) A disorder characterized by developmental delays, behavioral problems, and distinctive physical features.
  • SRY/male detection (Yp11.3) Used for determining male sex in genetic testing.
  • Steroid sulfatase deficiency (STS) (Xp22.3) A condition that can lead to various hormonal imbalances and developmental issues.
  • Williams (elastin) syndrome (7q11.23) A genetic condition that can cause cardiovascular disease, developmental delays, and unique personality traits.
  • Wolf-Hirschhorn (4p-) syndrome (4p16.3) A disorder caused by a deletion on the short arm of chromosome 4, leading to growth delays and intellectual disabilities.
  • Yq12 A region on the Y chromosome that may be analyzed for male-specific genetic conditions.

2. Procedure

The procedure for CPT® Code 88273 involves several critical steps to ensure accurate analysis of the chromosomal material. The first step is the collection of a blood sample or bone marrow aspirate, which is performed through a venipuncture or aspiration technique. This sample is then prepared for analysis, where the cells are cultured to increase their number and allow for detailed examination. Following this, chromosomal in situ hybridization is performed, which involves applying fluorescent probes that bind to specific regions of the chromosomes. This technique allows for the visualization of chromosomal structures and abnormalities under a fluorescence microscope. The analysis focuses on 10 to 30 cells, enabling the identification of microdeletions and other chromosomal abnormalities. The results are then interpreted by a qualified laboratory professional, who assesses the presence of any genetic markers or derivatives that may indicate specific genetic conditions.

3. Post-Procedure

After the procedure associated with CPT® Code 88273, the laboratory will process the samples and analyze the results. The expected recovery from the blood draw or bone marrow aspiration is typically quick, with patients advised to monitor the site for any signs of excessive bleeding or infection. Results from the chromosomal analysis may take several days to weeks, depending on the laboratory's processing times. Once the results are available, they will be communicated to the referring physician, who will discuss the findings with the patient and determine any necessary follow-up actions or treatments based on the identified genetic conditions. It is essential for healthcare providers to document the results accurately and consider any implications for the patient's health and family planning.

Short Descr CYTOGENETICS 10-30
Medium Descr MOLECULAR CYTOGENETICS CHRMOML ISH 10-30 CLL
Long Descr Molecular cytogenetics; chromosomal in situ hybridization, analyze 10-30 cells (eg, for microdeletions)
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 3
CCS Clinical Classification 235 - Other Laboratory
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
GA Waiver of liability statement issued as required by payer policy, individual case
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
Date
Action
Notes
2011-01-01 Changed Short description changed.
1999-01-01 Added First appearance in code book in 1999.
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