CPT® Code 88343

Code deleted, see 88344

Official Description

Immunohistochemistry or immunocytochemistry, each separately identifiable antibody per block, cytologic preparation, or hematologic smear; each additional separately identifiable antibody per slide (List separately in addition to code for primary procedure)

Common Language Description

Immunohistochemistry or immunocytochemistry is a laboratory technique used to identify specific antigens in cells or tissue samples by utilizing antibodies that are tailored to bind to those antigens. This process is crucial in the examination of cells found in tissue blocks, residual tissue fluid, or fine needle biopsies (FNB). The procedure involves preparing a tissue smear or sample on a glass slide, which is then fixed with a commercially available antibody. Following this, enzymes and/or special stains are applied to the slide, which enhances the visibility of the antigen-antibody reaction. The resulting characteristic changes observed in the cells can provide valuable information regarding the antigenic profile of morphologically undifferentiated cells, thereby assisting in the diagnosis of malignant neoplasms, commonly known as cancer. In this context, the coding for immunohistochemistry is structured such that CPT® Code 88342 is used for the first identifiable antibody per slide, while CPT® Code 88343 is designated for each additional separately identifiable antibody per slide, allowing for precise billing and documentation of the procedure performed.

1. Indications

Immunohistochemistry or immunocytochemistry is performed for various clinical indications, particularly when there is a need to identify specific antigens in tissue samples or cytologic preparations. The following are the explicitly provided indications for this procedure:

Diagnosis of Malignant Neoplasms - This procedure is essential in identifying cancerous cells by determining their antigenic profile, which aids in the diagnosis of malignancies.
Characterization of Tumors - It helps in characterizing tumors by identifying specific markers that can influence treatment decisions and prognostic evaluations.
Assessment of Undifferentiated Cells - The technique is utilized to assess morphologically undifferentiated cells, providing critical information for further diagnostic processes.

2. Procedure

The procedure for immunohistochemistry or immunocytochemistry involves several key steps that ensure accurate identification of antigens within the tissue samples. The following procedural steps are outlined:

Preparation of the Tissue Sample - The tissue sample is first prepared by creating a smear on a glass slide. This involves fixing the sample with a commercially available antibody to preserve the cellular structure and antigenicity.
Application of Antibodies - Once the sample is fixed, specific antibodies that are designed to bind to the target antigens are applied to the slide. This step is crucial as it allows for the identification of the antigens present in the cells.
Use of Enzymes and Stains - After the antibodies are applied, enzymes and/or special stains are introduced to the slide. These agents facilitate a visible reaction that highlights the presence of the antigens, making them easier to identify under a microscope.
Microscopic Examination - The final step involves examining the slide under a microscope to observe the characteristic changes in the cells. This examination helps in determining the antigenic profile and assists in diagnosing any malignancies present.

3. Post-Procedure

Post-procedure care for immunohistochemistry or immunocytochemistry typically involves ensuring that the slides are properly stored and documented for future reference. The results obtained from the procedure are analyzed and interpreted by pathologists, who will provide a report detailing the findings. This report is crucial for guiding further clinical decisions and treatment plans. Additionally, any necessary follow-up procedures or additional testing may be recommended based on the results of the immunohistochemical analysis.

Short Descr	IMMUNOHISTO ANTIBOD ADD SLID
Medium Descr	IMHISTOCHEM/CYTCHM EA ADDL ANTIBODY SLIDE
Long Descr	Immunohistochemistry or immunocytochemistry, each separately identifiable antibody per block, cytologic preparation, or hematologic smear; each additional separately identifiable antibody per slide (L
Status Code	Not Valid for Medicare Purposes
Global Days	ZZZ - Code Related to Another Service
PC/TC Indicator (26, TC)	1 - Diagnostic Tests for Radiology Services
Multiple Procedures (51)	0 - No payment adjustment rules for multiple procedures apply.
Bilateral Surgery (50)	0 - 150% payment adjustment for bilateral procedures does NOT apply.
Physician Supervisions	9 - Concept does not apply.
Assistant Surgeon (80, 82)	0 - Payment restriction for assistants at surgery applies to this procedure...
Co-Surgeons (62)	0 - Co-surgeons not permitted for this procedure.
Team Surgery (66)	0 - Team surgeons not permitted for this procedure.
Diagnostic Imaging Family	99 - Concept Does Not Apply
APC Status Indicator	Non-Covered Service, not paid under OPPS
Type of Service (TOS)	5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS)	T1H - Lab tests - other (non-Medicare fee schedule)
MUE	Not applicable/unspecified.