CPT® Code 88346

The CPT® Code 88346 refers to a laboratory procedure known as immunofluorescence, specifically for the initial single antibody stain performed on a specimen. This test is crucial for identifying the presence of specific antibodies within various types of specimens, which may include tissue sections, cultured cell lines, or individual cells. The process utilizes fluorescent staining techniques in conjunction with advanced microscopy methods, such as epifluorescence or confocal microscopy, to visualize the antibodies. In primary (direct) immunofluorescence, a single antibody is chemically linked to a fluorophore, allowing it to bind to the epitope region of the target antigen. When exposed to light, the fluorophore emits a specific wavelength that can be detected under the microscope, indicating the presence of the target antigen. Alternatively, secondary (indirect) immunofluorescence employs two antibodies: an unlabeled primary antibody that attaches to the target antigen and a labeled secondary antibody that binds to the primary antibody. This method can amplify the immunofluorescent signal, enhancing the detection of the target. The use of code 88346 is specifically for reporting the initial single antibody staining procedure, while code 88350 is designated for each additional single antibody stain performed on the same specimen.