CPT® Code 88373

Add-On Code

Morphometric analysis, in situ hybridization (quantitative or semi-quantitative), using computer-assisted technology, per specimen; each additional single probe stain procedure (List separately in addition to code for primary procedure)

Morphometric analysis, in situ hybridization (quantitative or semi-quantitative) is a specialized laboratory technique employed by pathologists and geneticists to visualize and analyze specific genes or segments of genes within cells. This method utilizes fluorescent tagging to detect genetic abnormalities, which may include translocations, deletions, duplications, amplifications, and inversions on chromosomes, as well as in hematologic cells and solid tumors. The process begins with fixing cells onto a slide, followed by treatment to denature the DNA, converting it into single strands. A short sequence of single-stranded DNA, known as a probe, is designed to match a specific target gene or genes and is tagged with fluorescent labels. This probe is then applied to the slide, where it hybridizes, or binds, to the complementary DNA strands. After hybridization, the slide undergoes washing to eliminate any unbound probe, ensuring that only specific interactions are detected. The final analysis is conducted using computer-assisted technology, which allows for precise identification and interpretation of any genetic abnormalities present. The CPT® Code 88373 is specifically designated for reporting each additional single probe stain procedure performed in conjunction with the primary procedure, which is reported using 88367. In cases where multiple probes are utilized simultaneously within the same staining procedure, the appropriate code to report is 88374.