CPT® Code 89253

Assisted embryo hatching, also known as assisted hatching (AH) or assisted zygote hatching (AZH), is a specialized procedure utilized in the field of reproductive medicine. This technique is designed to facilitate the implantation of an embryo into the uterine lining by aiding the embryo in breaking free from the zona pellucida, which is the protective outer shell surrounding the embryo. The zona pellucida plays a crucial role in the early stages of embryonic development, but in certain cases, it can become overly rigid or thick, hindering the embryo's ability to implant successfully. This procedure is particularly indicated for women aged 38 or older, as they often experience a more rigid zona pellucida due to age-related changes. Additionally, assisted embryo hatching may be recommended for patients with poor egg quantity and quality, poor embryo quality characterized by excessive fragmentation or slow cell division rates, or those who have previously experienced failures in in vitro fertilization (IVF). The process involves placing the embryo under a microscope and stabilizing it with a specialized holding pipette. A small hollow needle is then used to introduce an acidic solution against the zona pellucida, which digests a portion of this outer layer, creating a small opening. This opening aids the embryo in hatching and subsequently allows for a more effective implantation into the uterine lining. After the procedure, the embryo is carefully washed and returned to the culture medium in preparation for the embryo transfer procedure.