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Official Description

ACTH stimulation panel; for adrenal insufficiency This panel must include the following: Cortisol (82533 x 2)

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

An ACTH stimulation panel is a diagnostic test designed to evaluate the function of the adrenal glands, which are critical components of the endocrine system located above each kidney. This panel specifically assesses adrenal insufficiency, which can be categorized as either primary or secondary. Primary adrenal insufficiency may arise from conditions such as autoimmune adrenalitis, Addison's disease, congenital abnormalities, or tumors affecting the adrenal glands. In contrast, secondary adrenal insufficiency is often linked to dysfunctions of the pituitary gland or hypothalamus, which regulate hormone production. The test involves measuring cortisol levels, a hormone produced by the adrenal cortex, which plays a vital role in various bodily functions, including metabolism and stress response. The ACTH stimulation test is optimally conducted in the morning, between 8 and 9 a.m., after the patient has consumed a fat-free meal to ensure accurate baseline measurements. During the procedure, a blood sample is collected through venipuncture to determine the baseline cortisol level. Following this, cosyntropin (Cortosyn) is administered intravenously at a dose of 0.25 mg, and the patient is allowed to rest undisturbed for a period of 30 to 60 minutes. A second blood sample is then obtained through another venipuncture to measure the cortisol level after the stimulation. The serum or plasma samples are analyzed using a quantitative chemiluminescent immunoassay, providing critical information regarding adrenal gland function and potential insufficiency.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The ACTH stimulation panel is indicated for the assessment of adrenal gland function in patients suspected of having adrenal insufficiency. The following conditions may warrant the performance of this panel:

  • Primary Adrenal Insufficiency - Conditions such as autoimmune adrenalitis, Addison's disease, congenital abnormalities, or tumors affecting the adrenal glands.
  • Secondary Adrenal Insufficiency - Dysfunction of the pituitary gland or hypothalamus that impacts hormone regulation and adrenal function.

2. Procedure

The procedure for the ACTH stimulation panel involves several critical steps to ensure accurate assessment of adrenal function.

  • Step 1: Baseline Cortisol Measurement - The test begins with the collection of a blood sample through venipuncture to measure the baseline cortisol level. This sample is crucial as it establishes the initial hormone level before any stimulation occurs.
  • Step 2: Administration of Cosyntropin - Following the baseline measurement, cosyntropin (Cortosyn) is administered intravenously at a dosage of 0.25 mg. This synthetic form of adrenocorticotropic hormone (ACTH) stimulates the adrenal glands to produce cortisol.
  • Step 3: Rest Period - After the administration of cosyntropin, the patient is instructed to rest undisturbed for a duration of 30 to 60 minutes. This rest period is essential for allowing the adrenal glands to respond to the stimulation adequately.
  • Step 4: Post-Stimulation Cortisol Measurement - A second blood sample is collected through another venipuncture after the rest period. This sample is used to measure the cortisol level 30 to 60 minutes post-cosyntropin administration, providing insight into the adrenal glands' functional capacity.
  • Step 5: Laboratory Analysis - The serum or plasma samples obtained from both venipunctures are analyzed using a quantitative chemiluminescent immunoassay. This laboratory technique allows for precise measurement of cortisol levels, which is critical for diagnosing adrenal insufficiency.

3. Post-Procedure

After the completion of the ACTH stimulation panel, patients may resume normal activities unless otherwise directed by their healthcare provider. It is important to monitor for any adverse reactions to the cosyntropin injection, although such reactions are rare. The results of the cortisol measurements will be interpreted by the physician to determine the presence and type of adrenal insufficiency, guiding further management and treatment options as necessary.

Short Descr ACTH STIMULATION PANEL
Medium Descr ACTH STIMULATION PANEL ADRENAL INSUFFICIENCY
Long Descr ACTH stimulation panel; for adrenal insufficiency This panel must include the following: Cortisol (82533 x 2)
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 235 - Other Laboratory
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
CC Procedure code change (use 'cc' when the procedure code submitted was changed either for administrative reasons or because an incorrect code was filed)
GA Waiver of liability statement issued as required by payer policy, individual case
GC This service has been performed in part by a resident under the direction of a teaching physician
GW Service not related to the hospice patient's terminal condition
Q1 Routine clinical service provided in a clinical research study that is in an approved clinical research study
Q4 Service for ordering/referring physician qualifies as a service exemption
Date
Action
Notes
1994-01-01 Added First appearance in code book in 1994.
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