CPT® Code 85247

The CPT® Code 85247 refers to a laboratory test that performs a multimetric analysis of factor VIII and von Willebrand factor (VWF). This test is crucial in understanding the coagulation process, as factor VIII, also known as the antihemophilic factor, and VWF work together to form a unique plasma glycoprotein essential for normal blood clotting. VWF serves as the carrier protein for factor VIII, stabilizing its procoagulant activity, which is vital for effective hemostasis. In the bloodstream, VWF exists in two forms: one that circulates freely and another that is bound to the alpha granules of circulating platelets. The synthesis and release of plasma VWF occur in vascular endothelial cells, while platelet VWF is produced by megakaryocytes in the bone marrow. The multimetric analysis test encompasses several components, including the activity of factor VIII, the activity of VWF, and the VWF ristocetin cofactor activity. This comprehensive analysis is particularly ordered when there is a strong suspicion of inherited von Willebrand disease, a genetic disorder that affects blood clotting. To perform this test, a blood sample is collected through a venipuncture, which is separately reportable. The analysis is conducted on platelet-poor plasma using various methodologies such as electrophoresis, western blot, clotting assays, microlatex particle-mediated immunoassays, or platelet agglutination techniques.