CPT® Code 86835

Antibody to human leukocyte antigens (HLA), solid phase assays (eg, microspheres or beads, ELISA, Flow cytometry); semi-quantitative panel (eg, titer), HLA Class II

The human leukocyte antigen (HLA) system is a critical component of the major histocompatibility complex (MHC), which plays a vital role in the immune system's ability to recognize self from non-self. This system is essential for the identification of specific characteristics of an individual's cells and tissues, thereby enabling the body to defend itself against foreign substances, including microorganisms and non-self cells and tissues. The HLA system is divided into two main classes: Class I and Class II. The semi-quantitative analysis of HLA, particularly for Class II, is performed using various solid phase assays, such as microspheres or beads, enzyme-linked immunosorbent assay (ELISA), and flow cytometry. This testing is crucial for monitoring pre-transplant desensitization protocols for potential transplant recipients who have developed sensitization, as well as for assessing the treatment of antibody-mediated rejection following transplantation. The semi-quantitative panel with titer for HLA Class II is specifically designed to provide insights into the immune response and compatibility of transplant candidates. For HLA Class I testing, a different code, 86834, is utilized, while 86835 is designated for the semi-quantitative panel with titer for HLA Class II.