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Official Description

Culture, typing; immunofluorescent method, each antiserum

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The procedure described by CPT® Code 87140 involves the culture and typing of a specimen using the immunofluorescent method. This technique is utilized to identify specific antisera present in a blood serum sample. During the process, a sample of blood serum is first stained with a fluorescent dye, which allows for the visualization of the different antisera when examined under a microscope. The use of fluorescent dyes enhances the contrast of the antisera against the background, making it easier to distinguish between various types. This method is particularly valuable in clinical laboratories for diagnosing infections and determining the presence of specific antibodies in the serum, which can be critical for patient management and treatment decisions.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The immunofluorescent method for culture typing, as described by CPT® Code 87140, is indicated for various clinical scenarios where the identification of specific antisera is necessary. This procedure is commonly performed in the following situations:

  • Diagnosis of Infections The procedure is utilized to identify the presence of specific antibodies in response to infections, aiding in the diagnosis of various infectious diseases.
  • Serological Testing It is indicated for serological testing to determine immune responses to pathogens, which can be crucial for understanding a patient's immune status.
  • Monitoring Immune Disorders The test may be performed to monitor patients with immune disorders, helping to assess their immune function and response to therapies.

2. Procedure

The procedure for CPT® Code 87140 involves several key steps that ensure accurate identification of antisera in the blood serum sample. Each step is critical to the overall success of the immunofluorescent method.

  • Step 1: Sample Collection A blood sample is collected from the patient, typically through venipuncture. The collected serum is then separated from the blood cells by centrifugation, ensuring that only the serum component is used for testing.
  • Step 2: Staining with Fluorescent Dye The serum sample is treated with a specific fluorescent dye that binds to the target antisera. This staining process is essential as it allows the antisera to become visible under a fluorescence microscope.
  • Step 3: Microscopic Examination After staining, the sample is placed on a microscope slide and examined under a fluorescence microscope. The technician looks for specific patterns of fluorescence that indicate the presence of different antisera, which helps in typing the culture.
  • Step 4: Interpretation of Results The results are interpreted based on the fluorescence patterns observed. Each pattern corresponds to specific antisera, allowing for accurate typing and identification of the antibodies present in the serum.

3. Post-Procedure

Following the completion of the immunofluorescent culture typing procedure, the laboratory will typically provide a report detailing the findings. The results may indicate the presence or absence of specific antisera, which can inform further clinical decisions. There are generally no specific post-procedure care requirements for the patient, as the procedure is performed on a serum sample and does not involve any invasive techniques. However, it is essential for healthcare providers to discuss the results with the patient and consider any necessary follow-up testing or treatment based on the findings.

Short Descr CULTURE TYPE IMMUNOFLUORESC
Medium Descr CULTURE TYPING IMMUNOFLUORESCENT EACH ANTISERUM
Long Descr Culture, typing; immunofluorescent method, each antiserum
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 3
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
GP Services delivered under an outpatient physical therapy plan of care
GW Service not related to the hospice patient's terminal condition
GZ Item or service expected to be denied as not reasonable and necessary
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
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2001-01-01 Changed Code description changed.
Pre-1990 Added Code added.
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