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Official Description

Cytopathology, slides, cervical or vaginal (the Bethesda System); manual screening under physician supervision

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 88164 refers to the procedure of cytopathology specifically for cervical or vaginal specimens, commonly known as a Papanicolaou (PAP) smear. This test is integral in the early detection of cervical cancer and other abnormalities in cervical cells. The Bethesda System, which is the reporting framework utilized for this procedure, provides a standardized method for categorizing the results of the PAP smear. This system assesses the adequacy of the sample collected, identifies any signs of infection, and categorizes cellular changes into various classifications. These classifications include benign cellular changes, atypical squamous cells of undetermined significance (ASCUS), atypical glandular cells of undetermined significance (AGCUS), and various degrees of dysplasia or squamous intraepithelial lesions (SIL), which are further divided into low-grade (LGSIL) and high-grade (HGSIL) lesions, as well as carcinoma. During the procedure, cells are collected from the endocervix using a specialized instrument, such as a brush or stick, and then smeared onto a glass slide. The slide is subsequently fixed using either a spray or immersion method and is then covered with a coverslip. Once prepared, the slide is sent to a cytology laboratory where it undergoes staining to enhance visibility under a microscope. A trained technician, under the supervision of a physician, manually screens the slide for any abnormalities. This screening process involves identifying abnormal cells or clusters of cells that may indicate potential issues. The procedure described by CPT® Code 88164 specifically denotes a single manual screening performed by the technician, ensuring that the process is conducted under the oversight of a qualified physician. This careful approach is crucial for accurate diagnosis and subsequent management of any identified abnormalities.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The procedure associated with CPT® Code 88164 is indicated for the following conditions:

  • Cervical Cancer Screening This test is performed as part of routine screening for cervical cancer in women.
  • Detection of Abnormalities It is used to identify any abnormal cellular changes in the cervical or vaginal area that may require further investigation.
  • Monitoring of Previous Abnormal Results The procedure is indicated for patients who have had previous abnormal PAP smear results and require follow-up screening.
  • Assessment of Symptoms It may be indicated for patients presenting with symptoms such as abnormal vaginal bleeding or unusual discharge.

2. Procedure

The procedure for CPT® Code 88164 involves several key steps that ensure the accurate collection and analysis of cervical cells:

  • Step 1: Collection of Specimen The first step involves the collection of cervical cells using a specialized instrument, such as a brush or stick, which is inserted into the endocervical canal. This technique is designed to gather a representative sample of cells from the cervix.
  • Step 2: Preparation of the Slide Once the cells are collected, they are smeared onto a glass slide. The slide is then fixed using either a spray or immersion method to preserve the cellular structure for microscopic examination.
  • Step 3: Staining and Laboratory Processing The prepared slide is sent to a cytology laboratory where it undergoes staining. This staining process enhances the visibility of the cells under a microscope, allowing for better identification of any abnormalities.
  • Step 4: Manual Screening A trained technician, under the supervision of a physician, manually screens the stained slide using a microscope. During this screening, the technician looks for any abnormal cells or clusters that may indicate potential issues.
  • Step 5: Documentation and Reporting After the screening, the findings are documented and communicated to the treating physician. If any abnormalities are detected, the technician may recommend further testing or follow-up procedures.

3. Post-Procedure

After the procedure associated with CPT® Code 88164, the patient may be advised on several post-procedure considerations. Typically, there are no specific restrictions following the test, and patients can resume normal activities immediately. However, if any abnormalities are found during the screening, the pathologist may recommend additional follow-up procedures, such as a repeat PAP smear at a shorter interval, colposcopy, endocervical curettage, or biopsy to further investigate the findings. The results of the PAP smear are communicated to the treating physician, who will discuss the findings with the patient and determine the appropriate next steps based on the results.

Short Descr CYTOPATH TBS C/V MANUAL
Medium Descr CYTP SLIDES CERV/VAG MNL SCRN PHYSICIAN SUPV
Long Descr Cytopathology, slides, cervical or vaginal (the Bethesda System); manual screening under physician supervision
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 234 - Pathology

This is a primary code that can be used with these additional add-on codes.

88155 Female Edit Addon Code MPFS Status: Statutory exclusion (from MPFS, may be paid under other methodologies) APC Q4 PUB 100 CPT Assistant Article Cytopathology, slides, cervical or vaginal, definitive hormonal evaluation (eg, maturation index, karyopyknotic index, estrogenic index) (List separately in addition to code[s] for other technical and interpretation services)
GA Waiver of liability statement issued as required by payer policy, individual case
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
GZ Item or service expected to be denied as not reasonable and necessary
QW Clia waived test
Date
Action
Notes
2011-01-01 Changed Short description changed.
1999-01-01 Added First appearance in code book in 1999.
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