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Official Description

Oocyte identification from follicular fluid

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The procedure described by CPT® Code 89254 involves the identification of oocytes, or eggs, from follicular fluid. This process begins with the collection of follicular aspirates, which are obtained through a separate, reportable procedure. Once collected, the aspirates are placed into a test tube for further examination. The contents of this test tube are then distributed into a very thin layer across several Petri dishes, allowing for optimal visibility and examination. Each Petri dish is scrutinized under a stereo dissecting microscope, which is equipped with an illuminated base and a heated stage to facilitate the identification of oocytes. During this examination, the presence of oocytes is confirmed, and the maturation stage of each oocyte is assessed. Additionally, the condition of the surrounding coronal and cumulus cells is evaluated, as these factors are crucial in determining the viability and maturity of the oocytes. Notably, the presence of a single polar body is a key indicator of oocyte maturity. The identification process continues until all oocytes have been thoroughly examined, isolated, and documented, ensuring a comprehensive assessment of the follicular fluid's contents.

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