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This enzyme-linked immunosorbent assay (ELISA) test, identified by CPT® Code 0030T, is specifically designed to detect antibodies that are directed against prothrombin. Prothrombin is a protein that plays a crucial role in blood coagulation. In this test, prothrombin is coated onto irradiated plates, and a phosphatidylserine/prothrombin complex serves as the antigen. The process begins with the binding of the antibody, which is purified and attached to a solid phase. Following this, the antigen is introduced, allowing it to bind with the antibody, resulting in the formation of an antigen/antibody complex. Any unbound products are subsequently washed away to ensure specificity. A second detection antibody is then added, which binds to the antigen within the pre-formed complex. This creates a matrix that includes both the complex and the second antibody, which is then measured to determine the presence of antibodies. The test screens for immunoglobulin class antibodies, which are significant in diagnosing antiphospholipid syndrome, a condition associated with increased risk of thrombosis and pregnancy complications.
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The antiprothrombin (phospholipid cofactor) antibody test is indicated for the evaluation of patients suspected of having antiphospholipid syndrome. This condition is characterized by the presence of antiphospholipid antibodies, which can lead to an increased risk of thrombosis and complications during pregnancy. The test is particularly useful in patients who have experienced unexplained venous or arterial thrombosis, recurrent miscarriages, or other pregnancy-related complications.
The procedure for conducting the antiprothrombin antibody test involves several key steps to ensure accurate results. First, the prothrombin is coated onto irradiated plates, which serve as the solid phase for the assay. This coating is essential as it provides the antigen necessary for the binding process. Next, the purified antibody is introduced to the plates, allowing it to bind to the coated prothrombin. Following this initial binding, the phosphatidylserine/prothrombin complex is added to the wells, where it interacts with the bound antibodies to form an antigen/antibody complex. After allowing sufficient time for binding, any unbound products are washed away to eliminate background noise and enhance specificity. Subsequently, a second detection antibody is added to the wells. This detection antibody binds to the antigen within the already-formed complex, creating a stable matrix that can be measured. Finally, the amount of the second antibody is quantified, which reflects the presence of the antiprothrombin antibodies in the sample. This detailed procedural approach ensures that the test accurately screens for immunoglobulin class antibodies relevant to antiphospholipid syndrome.
After the completion of the antiprothrombin antibody test, there are no specific post-procedure care requirements mentioned. However, it is essential for healthcare providers to interpret the results in conjunction with clinical findings and other laboratory tests. Patients may be advised to follow up with their healthcare provider to discuss the results and any further diagnostic or therapeutic steps that may be necessary based on the findings of the test. Additionally, it is important to ensure that the results are documented accurately in the patient's medical record for future reference and continuity of care.
| Short Descr | ANTIPROTHROMBIN ANTIBODY | Medium Descr | ANTIPROTHROMBIN ANTIBODY EACH IG CLASS | Long Descr | Antiprothrombin (phospholipid cofactor) antibody, each Ig class | Status Code | Carriers Price the Code | Global Days | XXX - Global Concept Does Not Apply | PC/TC Indicator (26, TC) | 0 - Physician Service Code | Multiple Procedures (51) | 0 - No payment adjustment rules for multiple procedures apply. | Bilateral Surgery (50) | 0 - 150% payment adjustment for bilateral procedures does NOT apply. | Physician Supervisions | 09 - Concept does not apply. | Assistant Surgeon (80, 82) | 0 - Payment restriction for assistants at surgery applies to this procedure... | Co-Surgeons (62) | 0 - Co-surgeons not permitted for this procedure. | Team Surgery (66) | 0 - Team surgeons not permitted for this procedure. | Diagnostic Imaging Family | 99 - Concept Does Not Apply | APC Status Indicator | Discontinued Code | Type of Service (TOS) | 5 - Diagnostic Laboratory | Berenson-Eggers TOS (BETOS) | none | MUE | Not applicable/unspecified. | CCS Clinical Classification | 235 - Other Laboratory |
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| 2013-01-01 | Deleted | Code deleted, see 86849 |
| 2013-01-01 | Changed | Short and Medium Descriptors changed. |
| 2003-01-01 | Added | Code added. |
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