CPT® Code 0030T

Deleted

This enzyme-linked immunosorbent assay (ELISA) test, identified by CPT® Code 0030T, is specifically designed to detect antibodies that are directed against prothrombin. Prothrombin is a protein that plays a crucial role in blood coagulation. In this test, prothrombin is coated onto irradiated plates, and a phosphatidylserine/prothrombin complex serves as the antigen. The process begins with the binding of the antibody, which is purified and attached to a solid phase. Following this, the antigen is introduced, allowing it to bind with the antibody, resulting in the formation of an antigen/antibody complex. Any unbound products are subsequently washed away to ensure specificity. A second detection antibody is then added, which binds to the antigen within the pre-formed complex. This creates a matrix that includes both the complex and the second antibody, which is then measured to determine the presence of antibodies. The test screens for immunoglobulin class antibodies, which are significant in diagnosing antiphospholipid syndrome, a condition associated with increased risk of thrombosis and pregnancy complications.