© Copyright 2026 American Medical Association. All rights reserved.
CPT® Code 0058T refers to the procedure of cryopreservation of ovarian reproductive tissue. This process involves the careful harvesting of ovarian tissue, which is then preserved for future use in assisted reproductive technologies. The procedure begins with the collection of ovarian reproductive tissue during a separate, reportable surgical intervention. Once harvested, the tissue undergoes a thorough assessment to ensure its viability for future reproductive use. Following this evaluation, a series of specialized cryoprotectant solutions, designed specifically for ovarian tissue, are prepared to facilitate the preservation process. The ovarian tissue is then minced into smaller pieces to enhance the effectiveness of the cryoprotectants. It is sequentially immersed in these solutions, which contain increasing concentrations of cryoprotectant agents, to protect the cells during freezing. The actual freezing of the tissue can occur either by direct contact with the cryogenic medium or by placing the tissue in straws or vials before freezing. Each straw or vial is meticulously labeled with essential identifying information, such as the patient's name, identification number, and the contents of the container. The final step in the cryopreservation process involves gradually cooling the reproductive tissue to an extremely low temperature of -270 degrees centigrade using a controlled rate freezing chamber. This ensures that the tissue is preserved in optimal condition, either stored in the vapor phase or submerged in liquid nitrogen, until it is required for future assisted reproduction procedures.
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