CPT® Code 0280T

Deleted

Cell enumeration using immunologic selection and identification in fluid specimen (eg, circulating tumor cells in blood); interpretation and report

Cell enumeration using immunologic selection and identification in fluid specimens, such as circulating tumor cells in blood, represents an advanced technique for monitoring disease progression in patients diagnosed with certain types of cancer, including breast, colorectal, and prostate cancers. This innovative method involves a multistep process that utilizes specialized testing equipment to accurately identify and quantify tumor cells present in the bloodstream. Initially, a blood sample is collected using a designated collection kit, ensuring the integrity of the specimen. Following collection, the sample undergoes centrifugation to separate the plasma from the cellular components. The remaining cells are then mixed with a buffer solution and a reagent that is conjugated with a monoclonal antibody specifically designed to bind to epithelial cells. This selective binding allows for the separation of the epithelial cells from other cellular debris and leukocytes. A magnet is employed to facilitate this separation, attracting the bound epithelial cells while the unbound cells and excess fluid are discarded. Subsequently, the bound cells are subjected to a staining process using three distinct stains that help differentiate viable epithelial cells from any contaminating leukocytes or debris. Once stained, a magnet is again utilized to draw the labeled epithelial cells to the surface of a testing cartridge. A fluorescence-based microscopy system is then used to capture images of the cells at the cartridge's surface. A qualified technologist reviews these images to identify tumor cells based on their specific phenotype and the presence or absence of the designated stains. It is important to note that while CPT® Code 0279T covers the technical laboratory component of this cell enumeration process, CPT® Code 0280T pertains to the professional component, which includes the physician's review, interpretation of the results, and the generation of a written report detailing the findings.