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Official Description

Extractable nuclear antigen, antibody to, any method (eg, nRNP, SS-A, SS-B, Sm, RNP, Sc170, J01), each antibody

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 86235 refers to a laboratory test that measures the presence of antibodies to extractable nuclear antigens (ENAs) in a blood sample. These antibodies are proteins produced by the immune system that can mistakenly target the body's own tissues, which is a hallmark of autoimmune diseases. The test can detect various specific antibodies, including but not limited to nRNP, SS-A, SS-B, Sm, RNP, Sc170, and Jo-1. Elevated levels of these antibodies are often associated with conditions such as Sjogren's syndrome, polymyositis, systemic lupus erythematosus, and progressive systemic sclerosis. The procedure typically involves obtaining a blood sample through venipuncture, which is a separate reportable service. The testing can be performed using various methods, with semiquantitative multi-analyte fluorescent detection being a common approach. Each antibody detected is reported individually, allowing for a comprehensive assessment of the patient's autoimmune status.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The test coded as CPT® 86235 is indicated for the evaluation of autoimmune diseases. The specific conditions and symptoms for which this test may be performed include:

  • Sjogren's syndrome - A chronic autoimmune condition characterized by dry eyes and dry mouth due to the immune system attacking the glands that produce saliva and tears.
  • Polymyositis - An inflammatory disease causing muscle weakness, which is often associated with autoimmune processes.
  • Systemic lupus erythematosus - A systemic autoimmune disease that affects multiple organ systems and is characterized by a wide range of symptoms, including fatigue, joint pain, and skin rashes.
  • Progressive systemic sclerosis - Also known as scleroderma, this condition involves the hardening and tightening of the skin and connective tissues, often leading to serious complications.

2. Procedure

The procedure for CPT® 86235 involves several key steps to ensure accurate testing and reporting of antibodies to extractable nuclear antigens:

  • Step 1: Patient Preparation - Prior to the blood draw, the patient may be instructed to avoid certain medications or supplements that could interfere with test results. It is essential to ensure that the patient is adequately informed about the procedure.
  • Step 2: Venipuncture - A qualified healthcare professional performs a venipuncture to obtain a blood sample. This involves using a sterile needle to access a vein, typically in the arm, and collecting the blood into a designated tube. This step is crucial as it provides the specimen needed for testing.
  • Step 3: Laboratory Testing - The collected blood sample is sent to a laboratory where it undergoes testing for the presence of specific antibodies to extractable nuclear antigens. Various methods may be employed, including semiquantitative multi-analyte fluorescent detection, to accurately identify and quantify the antibodies present.
  • Step 4: Reporting Results - Once testing is complete, the laboratory generates a report detailing the presence and levels of each antibody tested. Each antibody, such as SSA (Ro) (ENA) Antibody, IgG; Jo-1 Antibody, IgG; and others, is reported individually, allowing for a comprehensive understanding of the patient's autoimmune profile.

3. Post-Procedure

After the procedure, patients may experience minor discomfort or bruising at the venipuncture site, which typically resolves quickly. There are no specific post-procedure care instructions required for this test, but patients should be advised to monitor the site for any unusual symptoms, such as excessive swelling or pain. The results of the test will be reviewed by the healthcare provider, who will discuss the findings with the patient and determine any necessary follow-up actions or treatments based on the results.

Short Descr NUCLEAR ANTIGEN ANTIBODY
Medium Descr EXTRACTABLE NUCLEAR ANTIGEN ANTIBODY ANY METHOD
Long Descr Extractable nuclear antigen, antibody to, any method (eg, nRNP, SS-A, SS-B, Sm, RNP, Sc170, J01), each antibody
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 10
CCS Clinical Classification 235 - Other Laboratory
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
Q4 Service for ordering/referring physician qualifies as a service exemption
GA Waiver of liability statement issued as required by payer policy, individual case
XS Separate structure, a service that is distinct because it was performed on a separate organ/structure
GW Service not related to the hospice patient's terminal condition
CC Procedure code change (use 'cc' when the procedure code submitted was changed either for administrative reasons or because an incorrect code was filed)
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
76 Repeat procedure or service by same physician or other qualified health care professional: it may be necessary to indicate that a procedure or service was repeated by the same physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 76 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
77 Repeat procedure by another physician or other qualified health care professional: it may be necessary to indicate that a basic procedure or service was repeated by another physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 77 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
95 Synchronous telemedicine service rendered via a real-time interactive audio and video telecommunications system: synchronous telemedicine service is defined as a real-time interaction between a physician or other qualified health care professional and a patient who is located at a distant site from the physician or other qualified health care professional. the totality of the communication of information exchanged between the physician or other qualified health care professional and the patient during the course of the synchronous telemedicine service must be of an amount and nature that would be sufficient to meet the key components and/or requirements of the same service when rendered via a face-to-face interaction. modifier 95 may only be appended to the services listed in appendix p. appendix p is the list of cpt codes for services that are typically performed face-to-face, but may be rendered via a real-time (synchronous) interactive audio and video telecommunications system.
AY Item or service furnished to an esrd patient that is not for the treatment of esrd
GV Attending physician not employed or paid under arrangement by the patient's hospice provider
GZ Item or service expected to be denied as not reasonable and necessary
PO Excepted service provided at an off-campus, outpatient, provider-based department of a hospital
QW Clia waived test
XE Separate encounter, a service that is distinct because it occurred during a separate encounter
XP Separate practitioner, a service that is distinct because it was performed by a different practitioner
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Pre-1990 Added Code added.
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