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Official Description

Intrinsic factor antibodies

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 86340 refers to the laboratory test for the detection of intrinsic factor antibodies (IFBA) in the blood. This test is crucial for identifying the presence of antibodies that block the action of intrinsic factor, a protein produced in the stomach that is essential for the absorption of vitamin B12. Vitamin B12 is vital for the production of red blood cells and the maintenance of the nervous system. The intrinsic factor binds to free vitamin B12 and transports it to specific receptors in the ileal mucosa, facilitating its absorption in the small intestine. When intrinsic factor is absent, it can lead to a condition known as pernicious anemia (PA), which is characterized by the inability to absorb vitamin B12 effectively. The presence of intrinsic factor antibodies is significant as they are found in approximately 50% of individuals diagnosed with pernicious anemia. However, these antibodies may also be present in patients with other autoimmune conditions, such as autoimmune thyroid disease, type 1 diabetes mellitus, and rheumatoid arthritis. The test is performed by obtaining a blood sample through a venipuncture, which is a procedure that can be reported separately. The serum obtained from the blood sample is then analyzed using a qualitative enzyme-linked immunosorbent assay (ELISA) to determine the presence of these antibodies.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The CPT® Code 86340 is indicated for the following conditions and symptoms:

  • Pernicious Anemia (PA) - A condition characterized by the inability to absorb vitamin B12 due to a lack of intrinsic factor.
  • Autoimmune Thyroid Disease - A group of disorders where the immune system attacks the thyroid gland, potentially leading to the presence of intrinsic factor antibodies.
  • Type 1 Diabetes Mellitus - An autoimmune condition that may be associated with the presence of intrinsic factor antibodies.
  • Rheumatoid Arthritis - A chronic inflammatory disorder that can also show the presence of intrinsic factor antibodies.

2. Procedure

The procedure for testing intrinsic factor antibodies involves several key steps:

  • Step 1: Patient Preparation - The patient may be instructed to avoid certain medications or supplements that could interfere with the test results. It is essential to ensure that the patient is adequately informed about the procedure and its purpose.
  • Step 2: Venipuncture - A qualified healthcare professional performs a venipuncture to obtain a blood sample. This involves inserting a needle into a vein, typically in the arm, to draw blood into a collection tube. The procedure is performed using sterile techniques to minimize the risk of infection.
  • Step 3: Serum Separation - After the blood sample is collected, it is processed to separate the serum from the cellular components. This is typically done by centrifugation, which spins the blood at high speeds to separate the different components based on their density.
  • Step 4: Testing - The serum is then subjected to a qualitative enzyme-linked immunosorbent assay (ELISA). This laboratory technique is used to detect the presence of intrinsic factor antibodies by using specific antigens that bind to the antibodies if they are present in the serum.
  • Step 5: Result Interpretation - The results of the ELISA are analyzed to determine whether intrinsic factor antibodies are present. A positive result indicates the presence of these antibodies, which may suggest an underlying condition such as pernicious anemia or other autoimmune disorders.

3. Post-Procedure

After the procedure, the patient may experience minor discomfort or bruising at the venipuncture site, which typically resolves quickly. There are no specific post-procedure care instructions required for this test, but patients should be advised to report any unusual symptoms or prolonged discomfort. The results of the test will be communicated to the healthcare provider, who will interpret the findings in the context of the patient's overall clinical picture and may recommend further evaluation or treatment based on the results.

Short Descr INTRINSIC FACTOR ANTIBODY
Medium Descr INTRINSIC FACTOR ANTIBODIES
Long Descr Intrinsic factor antibodies
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 235 - Other Laboratory
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
77 Repeat procedure by another physician or other qualified health care professional: it may be necessary to indicate that a basic procedure or service was repeated by another physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 77 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
GA Waiver of liability statement issued as required by payer policy, individual case
GW Service not related to the hospice patient's terminal condition
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
GZ Item or service expected to be denied as not reasonable and necessary
Q6 Service furnished under a fee-for-time compensation arrangement by a substitute physician or by a substitute physical therapist furnishing outpatient physical therapy services in a health professional shortage area, a medically underserved area, or a rural area
Date
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Pre-1990 Added Code added.
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