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Official Description

Infectious agent detection by nucleic acid (DNA or RNA); Bartonella henselae and Bartonella quintana, amplified probe technique

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 87471 refers to a laboratory test designed for the detection of the infectious agents Bartonella henselae and Bartonella quintana through the use of nucleic acid analysis, specifically targeting DNA or RNA. Bartonella is classified as a small, gram-negative bacillus that poses challenges in terms of isolation and culture in laboratory settings. The significance of this test lies in its ability to utilize nucleic acid probes, which facilitate the rapid identification of the DNA or RNA of the Bartonella species without the necessity for traditional culture methods. B. henselae is known to cause cat scratch disease (CSD), a condition that can result in severe disseminated infections, while B. quintana is linked to trench fever and bacillary angiomatosis, particularly in patients with HIV. The amplified probe technique employed in this test enhances the sensitivity of the assay by exponentially multiplying the target sequences of Bartonella DNA or RNA into millions of copies, thereby allowing for more accurate detection. Common amplification methods include polymerase chain reaction (PCR) and reverse transcriptase polymerase chain reaction (RT-PCR). Following amplification, the replicated sequences are identified using DNA probes that are labeled with either fluorescent or chemiluminescent markers, which specifically bind to the target sequences. Additionally, nucleic acid detection with quantification, as indicated by CPT® Code 87472, provides an assessment of the quantity of microorganisms present by employing quantitative or real-time PCR techniques to amplify the isolated nucleic acid segments and generate detailed reports on the absolute or relative amounts of the identified nucleic acid sequences.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The CPT® Code 87471 is indicated for the detection of specific infectious agents, particularly in the following scenarios:

  • Cat Scratch Disease (CSD) - This condition is caused by Bartonella henselae, which can lead to severe disseminated infections, particularly in immunocompromised individuals.
  • Trench Fever - Associated with Bartonella quintana, this illness is characterized by recurrent fever and is often seen in populations with poor hygiene or crowded living conditions.
  • Bacillary Angiomatosis - This condition, which can occur in patients with HIV, is linked to Bartonella quintana and presents with vascular lesions that can be mistaken for other conditions.

2. Procedure

The procedure for CPT® Code 87471 involves several critical steps to ensure accurate detection of the target infectious agents:

  • Sample Collection - A biological sample, such as blood or tissue, is collected from the patient, which may contain the DNA or RNA of the Bartonella species.
  • Nucleic Acid Extraction - The nucleic acids are extracted from the collected sample using specific laboratory techniques that isolate the DNA or RNA from other cellular components.
  • Amplification of Target Sequences - The extracted nucleic acids undergo an amplification process, typically using polymerase chain reaction (PCR) or reverse transcriptase polymerase chain reaction (RT-PCR). This step exponentially increases the number of copies of the target Bartonella DNA or RNA, enhancing the sensitivity of the test.
  • Detection of Amplified Sequences - Following amplification, the replicated sequences are identified using nucleic acid probes that are labeled with fluorescent or chemiluminescent markers. These probes specifically bind to the target sequences, allowing for their detection.
  • Analysis and Reporting - The results of the test are analyzed, and a report is generated that indicates the presence or absence of the infectious agents, along with any quantification of the nucleic acid detected, if applicable.

3. Post-Procedure

After the procedure associated with CPT® Code 87471, the laboratory will typically provide a report detailing the findings. Clinicians may use this information to guide further diagnostic or therapeutic decisions. There are no specific post-procedure care instructions mentioned; however, it is essential for healthcare providers to monitor the patient for any symptoms related to the infections being tested. Follow-up consultations may be necessary to discuss the results and any subsequent treatment options based on the findings of the test.

Short Descr BARTONELLA DNA AMP PROBE
Medium Descr IADNA BARTONELLA AMPLIFIED PROBE TECHNIQUE
Long Descr Infectious agent detection by nucleic acid (DNA or RNA); Bartonella henselae and Bartonella quintana, amplified probe technique
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
GW Service not related to the hospice patient's terminal condition
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
T7 Right foot, third digit
T5 Right foot, great toe
T9 Right foot, fifth digit
T1 Left foot, second digit
XS Separate structure, a service that is distinct because it was performed on a separate organ/structure
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
GA Waiver of liability statement issued as required by payer policy, individual case
GV Attending physician not employed or paid under arrangement by the patient's hospice provider
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
LT Left side (used to identify procedures performed on the left side of the body)
RT Right side (used to identify procedures performed on the right side of the body)
T2 Left foot, third digit
T3 Left foot, fourth digit
T4 Left foot, fifth digit
T8 Right foot, fourth digit
Date
Action
Notes
2011-01-01 Changed Short description changed.
1998-01-01 Added First appearance in code book in 1998.
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