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Official Description

Infectious agent detection by nucleic acid (DNA or RNA); Streptococcus, group B, amplified probe technique

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 87653 refers to the detection of infectious agents through nucleic acid testing, specifically targeting Streptococcus, group B, using an amplified probe technique. This method is a significant advancement in diagnostic microbiology, providing a reliable alternative to traditional culturing methods. Nucleic acid detection focuses on identifying the genetic material—either DNA or RNA—of the infectious agent, which allows for the detection of much lower levels of the organism, sometimes down to a single cell. The process involves the release of the infectious agent's nucleic acids from the cells, followed by their extraction through a technique known as nucleic acid hybridization. This technique utilizes a specially designed probe, which consists of laboratory-prepared complementary strands of nucleic acid that are often labeled with chemical fluorescence. These probes are engineered to bind specifically to the target DNA, forming stable double-stranded complexes or hybrids. The amplified probe technique enhances the sensitivity of the test, enabling more direct and qualitative detection of the infectious agent. This is achieved through methods such as polymerase chain reaction (PCR), which enzymatically replicates specific target sequences within the DNA, resulting in exponential amplification of the genetic material. In this process, amplification primers—short strands of DNA that are complementary to the target DNA—are used alongside the probe. These primers attach to the target DNA at designated points, allowing the polymerase enzyme to copy the region and synthesize new DNA, thereby increasing the amount of detectable genetic material in the sample.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The CPT® Code 87653 is indicated for the detection of Streptococcus, group B, in patients who may present with symptoms or conditions associated with this infectious agent. The following are specific indications for performing this procedure:

  • Pregnant Women Screening for group B Streptococcus (GBS) is crucial as it can lead to serious infections in newborns if transmitted during delivery.
  • Neonates Evaluation of newborns who may exhibit signs of infection, particularly if the mother was positive for GBS during pregnancy.
  • Immunocompromised Patients Testing in individuals with weakened immune systems who are at higher risk for severe infections caused by Streptococcus, group B.

2. Procedure

The procedure for CPT® Code 87653 involves several critical steps to ensure accurate detection of the infectious agent. The following outlines the procedural steps:

  • Sample Collection A specimen is collected from the patient, which may include vaginal, rectal, or other relevant samples, depending on the clinical scenario. Proper collection techniques are essential to avoid contamination and ensure the integrity of the sample.
  • Nucleic Acid Extraction The collected sample undergoes a process to release the nucleic acids from the cells. This involves lysing the cells to free the DNA or RNA, which is then purified to remove any inhibitors that could affect the subsequent amplification process.
  • Hybridization with Probes The extracted nucleic acids are then subjected to hybridization with specific probes designed to bind to the target sequences of the Streptococcus, group B DNA or RNA. This step is critical for ensuring that only the relevant genetic material is detected.
  • Amplification Following hybridization, an amplification process, such as polymerase chain reaction (PCR), is employed. This involves the use of amplification primers that bind to the target DNA, allowing the polymerase enzyme to replicate the DNA, thereby increasing the quantity of the target sequence exponentially.
  • Detection The final step involves detecting the amplified DNA or RNA, often using fluorescence or other methods to visualize the presence of the target nucleic acid. This provides a qualitative result indicating whether Streptococcus, group B, is present in the sample.

3. Post-Procedure

After the completion of the procedure associated with CPT® Code 87653, the results are typically available within a short timeframe, allowing for timely clinical decision-making. It is essential for healthcare providers to interpret the results in conjunction with clinical findings and patient history. If the test is positive for Streptococcus, group B, appropriate management and treatment protocols should be initiated, particularly in pregnant women to prevent transmission to the newborn. Additionally, healthcare providers should ensure that proper documentation of the procedure and results is maintained for compliance and billing purposes.

Short Descr STREP B DNA AMP PROBE
Medium Descr IADNA STREPTOCOCCUS GROUP B AMPLIFIED PROBE TQ
Long Descr Infectious agent detection by nucleic acid (DNA or RNA); Streptococcus, group B, amplified probe technique
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
GW Service not related to the hospice patient's terminal condition
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
XS Separate structure, a service that is distinct because it was performed on a separate organ/structure
GV Attending physician not employed or paid under arrangement by the patient's hospice provider
GZ Item or service expected to be denied as not reasonable and necessary
T5 Right foot, great toe
T3 Left foot, fourth digit
RT Right side (used to identify procedures performed on the right side of the body)
T4 Left foot, fifth digit
T2 Left foot, third digit
T9 Right foot, fifth digit
T1 Left foot, second digit
LT Left side (used to identify procedures performed on the left side of the body)
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
56 Preoperative management only: when 1 physician or other qualified health care professional performed the preoperative care and evaluation and another performed the surgical procedure, the preoperative component may be identified by adding modifier 56 to the usual procedure number.
58 Staged or related procedure or service by the same physician or other qualified health care professional during the postoperative period: it may be necessary to indicate that the performance of a procedure or service during the postoperative period was: (a) planned or anticipated (staged); (b) more extensive than the original procedure; or (c) for therapy following a surgical procedure. this circumstance may be reported by adding modifier 58 to the staged or related procedure. note: for treatment of a problem that requires a return to the operating/procedure room (eg, unanticipated clinical condition), see modifier 78.
76 Repeat procedure or service by same physician or other qualified health care professional: it may be necessary to indicate that a procedure or service was repeated by the same physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 76 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
77 Repeat procedure by another physician or other qualified health care professional: it may be necessary to indicate that a basic procedure or service was repeated by another physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 77 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
79 Unrelated procedure or service by the same physician or other qualified health care professional during the postoperative period: the individual may need to indicate that the performance of a procedure or service during the postoperative period was unrelated to the original procedure. this circumstance may be reported by using modifier 79. (for repeat procedures on the same day, see modifier 76.)
GA Waiver of liability statement issued as required by payer policy, individual case
GF Non-physician (e.g. nurse practitioner (np), certified registered nurse anesthetist (crna), certified registered nurse (crn), clinical nurse specialist (cns), physician assistant (pa)) services in a critical access hospital
KX Requirements specified in the medical policy have been met
Q4 Service for ordering/referring physician qualifies as a service exemption
QJ Services/items provided to a prisoner or patient in state or local custody, however the state or local government, as applicable, meets the requirements in 42 cfr 411.4 (b)
QW Clia waived test
SA Nurse practitioner rendering service in collaboration with a physician
T6 Right foot, second digit
T7 Right foot, third digit
T8 Right foot, fourth digit
TA Left foot, great toe
XE Separate encounter, a service that is distinct because it occurred during a separate encounter
XP Separate practitioner, a service that is distinct because it was performed by a different practitioner
Date
Action
Notes
2011-01-01 Changed Short description changed.
2007-01-01 Added First appearance in code book in 2007.
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