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Official Description

Infectious agent detection by nucleic acid (DNA or RNA); hepatitis G, amplified probe technique

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 87526 refers to a laboratory test designed for the detection of the hepatitis G virus (HGV), also known as GB virus-C (GBV-C), through the use of nucleic acid amplification techniques. This test specifically targets the viral DNA or RNA, allowing for the identification of the infectious agent in a blood sample. Hepatitis G infection is typically asymptomatic and does not usually lead to significant liver inflammation; however, it can occur alongside other viral infections that may cause acute or chronic hepatitis. The transmission of HGV primarily occurs through exposure to infected blood and blood products, from mother to infant during childbirth, and through sexual contact with an infected individual. The testing process involves a direct probe test, which identifies a unique nucleic acid sequence known as the target sequence of the hepatitis G virus, if present in the sample. This is achieved by using a probe that is labeled with either fluorescent or chemiluminescent markers. The blood sample is treated to release nucleic acids from the virus, and the labeled probe binds specifically to the target sequence, forming a stable hybrid. In the amplified probe technique, the sensitivity of the assay is significantly enhanced by exponentially multiplying the target sequence of the hepatitis G virus DNA or RNA into millions of copies. The most prevalent amplification methods include polymerase chain reaction (PCR) and reverse transcriptase polymerase chain reaction (RT-PCR). Following amplification, the replicated sequences are identified using labeled DNA probes. Additionally, nucleic acid detection with quantification can be performed to assess the quantity of microorganisms present, often utilizing quantitative or real-time PCR to provide detailed reports on the absolute or relative amounts of the nucleic acid sequence detected.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The CPT® Code 87526 is indicated for the detection of the hepatitis G virus (HGV) in patients who may be at risk for infection. The following conditions and situations warrant the use of this test:

  • Asymptomatic Infection Patients who are asymptomatic but may have been exposed to HGV through blood transfusions, intravenous drug use, or sexual contact with an infected individual.
  • Co-infection Assessment Individuals who are co-infected with other hepatitis viruses, such as hepatitis B or C, where the presence of HGV may complicate the clinical picture.
  • Blood Donation Screening Screening of blood donors to ensure the safety of blood products and prevent transmission of HGV through transfusions.
  • Maternal Screening Testing of pregnant women who may have been exposed to HGV to assess the risk of vertical transmission to the infant.

2. Procedure

The procedure for CPT® Code 87526 involves several critical steps to ensure accurate detection of the hepatitis G virus. The following outlines the procedural steps:

  • Sample Collection A blood sample is collected from the patient, which serves as the source for nucleic acid extraction. Proper collection techniques are essential to avoid contamination and ensure the integrity of the sample.
  • Nucleic Acid Extraction The collected blood sample undergoes a treatment process to release nucleic acids from any present hepatitis G virus. This step is crucial as it prepares the sample for the subsequent amplification and detection phases.
  • Amplification of Target Sequence The extracted nucleic acids are subjected to an amplification process, typically using polymerase chain reaction (PCR) or reverse transcriptase polymerase chain reaction (RT-PCR). This step exponentially increases the quantity of the target sequence, enhancing the sensitivity of the test.
  • Hybridization with Labeled Probes After amplification, the replicated sequences are hybridized with labeled DNA probes that specifically bind to the target sequence of the hepatitis G virus. This binding forms stable hybrids that can be detected in the next step.
  • Detection and Analysis The final step involves detecting the labeled hybrids, which indicates the presence of the hepatitis G virus in the sample. The results are then analyzed to determine the presence and quantity of the virus, providing critical information for clinical decision-making.

3. Post-Procedure

After the completion of the procedure associated with CPT® Code 87526, the laboratory will analyze the results and generate a report detailing the presence of the hepatitis G virus. The results may indicate whether the virus is detected and, if applicable, the quantity of viral nucleic acid present in the sample. Clinicians will use this information to guide further patient management, which may include monitoring for co-infections, counseling on transmission risks, and determining the need for additional testing or treatment. It is important for healthcare providers to communicate the results to the patient and discuss any necessary follow-up actions based on the findings.

Short Descr HEPATITIS G DNA AMP PROBE
Medium Descr IADNA HEPATITIS G AMPLIFIED PROBE TECHNIQUE
Long Descr Infectious agent detection by nucleic acid (DNA or RNA); hepatitis G, amplified probe technique
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
Date
Action
Notes
2011-01-01 Changed Short description changed.
1998-01-01 Added First appearance in code book in 1998.
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