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Official Description

Infectious agent detection by nucleic acid (DNA or RNA); Bartonella henselae and Bartonella quintana, quantification

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 87472 refers to a laboratory test designed for the detection of the infectious agents Bartonella henselae and Bartonella quintana through the analysis of nucleic acids, specifically DNA or RNA. Bartonella is classified as a small, gram-negative bacillus that poses challenges in terms of isolation and culture in traditional laboratory settings. The use of nucleic acid probes facilitates the rapid identification of these specific Bartonella species by targeting their genetic material without the necessity for culturing the organisms. Bartonella henselae is primarily known for causing cat scratch disease (CSD), which can result in severe disseminated infections, while Bartonella quintana is linked to trench fever and bacillary angiomatosis, particularly in patients with HIV. The procedure employs advanced techniques such as polymerase chain reaction (PCR) or reverse transcriptase polymerase chain reaction (RT-PCR) to amplify the target sequences of Bartonella DNA or RNA, significantly enhancing the assay's sensitivity. This amplification process generates millions of copies of the target nucleic acid, which are then detected using DNA probes that are labeled with either fluorescent or chemiluminescent markers, ensuring specificity to the target sequence. Furthermore, the quantification aspect of this test allows for an assessment of the microbial load present in the sample, often utilizing quantitative or real-time PCR methods to provide detailed reports on the absolute or relative amounts of the detected nucleic acid sequences.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The CPT® Code 87472 is indicated for the detection of specific infectious agents, particularly in the context of the following conditions:

  • Cat Scratch Disease (CSD) - This condition is caused by Bartonella henselae and can lead to serious disseminated infections.
  • Trench Fever - Associated with Bartonella quintana, this condition is characterized by recurrent fever and is often seen in individuals with compromised immune systems.
  • Bacillary Angiomatosis - This is a condition linked to Bartonella quintana, particularly affecting patients with HIV, leading to vascular lesions.

2. Procedure

The procedure for CPT® Code 87472 involves several critical steps to ensure accurate detection and quantification of the target nucleic acids. The process begins with the collection of a specimen, which may include blood or tissue samples from the patient suspected of having an infection caused by Bartonella species. Once the sample is obtained, it undergoes a nucleic acid extraction process to isolate the DNA or RNA present in the specimen. This step is crucial as it prepares the genetic material for amplification.

  • Nucleic Acid Amplification - Following extraction, the nucleic acid is subjected to an amplification technique, typically polymerase chain reaction (PCR) or reverse transcriptase polymerase chain reaction (RT-PCR). This process exponentially increases the quantity of the target Bartonella DNA or RNA, allowing for enhanced sensitivity in detection.
  • Detection of Amplified Sequences - After amplification, the replicated nucleic acid sequences are identified using specific DNA probes that are labeled with fluorescent or chemiluminescent markers. These probes bind exclusively to the target sequences, facilitating accurate identification.
  • Quantification - The final step involves quantifying the amplified nucleic acid sequences. This is often achieved through quantitative or real-time PCR, which provides a detailed report on the absolute or relative amounts of the detected nucleic acid, offering insights into the microbial load present in the sample.

3. Post-Procedure

Post-procedure care for patients undergoing testing with CPT® Code 87472 typically involves monitoring for any potential side effects related to the specimen collection, such as minor discomfort or bruising at the site of blood draw. The results of the test are usually communicated to the healthcare provider, who will interpret the findings in the context of the patient's clinical presentation. Depending on the quantification results, further diagnostic or therapeutic measures may be considered to address the identified infection. It is essential for healthcare providers to ensure that patients understand the implications of the test results and any necessary follow-up actions.

Short Descr BARTONELLA DNA QUANT
Medium Descr IADNA BARTONELLA HENSELAE&QUINTANA QUANTJ
Long Descr Infectious agent detection by nucleic acid (DNA or RNA); Bartonella henselae and Bartonella quintana, quantification
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
Date
Action
Notes
2011-01-01 Changed Short description changed.
1998-01-01 Added First appearance in code book in 1998.
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